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Addgene

pRK5-p18-FLAG
(Plasmid #42331)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42331 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone manufacturer
    BD PharMingen
  • Backbone size w/o insert (bp) 4661
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p18
  • Alt name
    LAMTOR1
  • Alt name
    late endosomal/lysosomal adaptor, MAPK and MTOR activator 1
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_017907.2 NP_060377.1
  • Entrez Gene
    LAMTOR1 (a.k.a. C11orf59, PDRO, Ragulator1, p18, p27RF-Rho)
  • Promoter CMV, SP6
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (destroyed during cloning)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-p18-FLAG was a gift from David Sabatini (Addgene plasmid # 42331 ; http://n2t.net/addgene:42331 ; RRID:Addgene_42331)

  • For your References section:

    Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM. Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. 10.1016/j.cell.2012.07.032 PubMed 22980980
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