pGEX VAMP7 (1-188)
(Plasmid #42315)

• Depositing Lab: Thierry Galli
• Publication: Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Backbone

• Vector backbone: pGEX 4T
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VAMP7 Cytoplasmic domain (1-181)
• Alt name: VAMP7
• Alt name: vesicle-associated membrane protein 7
• Alt name: TI-VAMP/Synaptobrevin-like (Sybl 1)
• Species: H. sapiens (human)
• Mutation: Δ181-220
• GenBank ID: NM_005638.5 NP_005629.1
• Entrez Gene: VAMP7 (a.k.a. SYBL1, TI-VAMP, TIVAMP, VAMP-7)
• Promoter: Ptac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Alternate plasmid name: cytosolic domain-TIVAMP (Cyt-TIVAMP)

Although listed as 1-188 in the publication, this construct contains residues 1-181 and the truncation does not affect plasmid function.