pEGFP VAMP4
(Plasmid #42313)

• Depositing Lab: Thierry Galli
• Publication: Mallard et al J Cell Biol. 2002 Feb 18;156(4):653-64. Epub 2002 Feb 11.

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VAMP 4
• Alt name: vesicle-associated membrane protein 4
• Species: H. sapiens (human)
• GenBank ID: NM_003762.4 NP_003753.2
• Entrez Gene: VAMP4 (a.k.a. VAMP-4, VAMP24)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: EGFP-C; CMV-F
• 3′ sequencing primer: SV40-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry