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Addgene

pEGFP VARP
(Plasmid #42312)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42312 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VARP
  • Alt name
    ANKRD27
  • Alt name
    ankyrin repeat domain 27 (VPS9 domain)
  • Alt name
    PP12899
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_032139.2 NP_115515.2
  • Entrez Gene
    ANKRD27 (a.k.a. PP12899, VARP)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N; SV40-pA-R
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Full length human Varp coding sequence was subcloned from pEntr221-Varp (RZPD, Berlin, Germany).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP VARP was a gift from Thierry Galli (Addgene plasmid # 42312 ; http://n2t.net/addgene:42312 ; RRID:Addgene_42312)

  • For your References section:

    Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth. Burgo A, Sotirakis E, Simmler MC, Verraes A, Chamot C, Simpson JC, Lanzetti L, Proux-Gillardeaux V, Galli T. EMBO Rep. 2009 Oct;10(10):1117-24. doi: 10.1038/embor.2009.186. Epub 2009 Sep 11. 10.1038/embor.2009.186 PubMed 19745841
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