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Addgene

pEGFP VAMP8
(Plasmid #42311)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42311 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VAMP 8
  • Alt name
    vesicle-associated membrane protein 8
  • Alt name
    EDB
  • Alt name
    Endobrevin
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_003761.4 NP_003752.2
  • Entrez Gene
    VAMP8 (a.k.a. EDB, VAMP-8)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer EGFP-C; CMV-F
  • 3′ sequencing primer SV40-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP VAMP8 was a gift from Thierry Galli (Addgene plasmid # 42311 ; http://n2t.net/addgene:42311 ; RRID:Addgene_42311)

  • For your References section:

    Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment. Paumet F, Le Mao J, Martin S, Galli T, David B, Blank U, Roa M. J Immunol. 2000 Jun 1;164(11):5850-7. PubMed 10820264
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