pEGFP VAMP2
(Plasmid #42308)

• Depositing Lab: Thierry Galli
• Publication: Martinez-Arca et al Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):9011-6. Epub 2003 Jul 9.

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VAMP 2
• Alt name: vesicle-associated membrane protein 2
• Alt name: SYB2
• Alt name: Synaptobrevin 2
• GenBank ID:
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EGFP-C; CMV-F
• 3′ sequencing primer: SV40-pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Rat Syb2 cDNA from Richard H. Scheller (Stanford University, Stanford, California)
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The V8A & T116S substitutions found in the Addgene sequence correspond to polymorphisms and have no functional impact.