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Addgene

pHYDuet93
(Plasmid #42303)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42303 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSKDuet16
  • Backbone size w/o insert (bp) 3992
  • Total vector size (bp) 4392
  • Modifications to backbone
    introduced stop codon by inversion PCR to create NpuDnaEdC6-stop-
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NpuDnaEdC6
  • Alt name
    N-intein of NpuDnaEC6 split intein
  • Alt name
    split intein
  • Species
    Nostoc punctiforme
  • Insert Size (bp)
    400
  • Promoter T7
  • Tag / Fusion Protein
    • H6-GB1 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHYDuet93 was a gift from Hideo Iwai (Addgene plasmid # 42303 ; http://n2t.net/addgene:42303 ; RRID:Addgene_42303)

  • For your References section:

    Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification. Oeemig JS, Aranko AS, Djupsjobacka J, Heinamaki K, Iwai H. FEBS Lett. 2009 May 6;583(9):1451-6. doi: 10.1016/j.febslet.2009.03.058. Epub 2009 Apr 1. 10.1016/j.febslet.2009.03.058 PubMed 19344715
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