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Addgene

pCGMbX
(Plasmid #42300)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42300 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT/BiP/V5-His A
  • Backbone manufacturer
    Invitrogen (Life Technologies)
  • Backbone size (bp) 3600
  • Modifications to backbone
    Expression cassette inserted upstream of the metallothionein promoter, enabling constitutive expression of eGFP.
  • Vector type
    Insect Expression
  • Promoter metallothonein
  • Tags / Fusion Proteins
    • BiP signal sequence (N terminal on insert)
    • V5-epitope (C terminal on backbone)
    • His6 (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer catctcagtgcaactaaa
  • 3′ sequencing primer TGTCTGGATCCTCGATCG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid was generated from a combination of pCo-Blast (Invitrogen), pMT/BiP/V5-His A (Invitrogen), and pMT-eGFP by Vladimir Vigdorovich.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCGMbX was a gift from Steven Almo (Addgene plasmid # 42300 ; http://n2t.net/addgene:42300 ; RRID:Addgene_42300)
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