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Tol2-ftyrp>TRAP
(Plasmid #42299)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42299 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT2KXIG
  • Backbone size w/o insert (bp) 6140
  • Total vector size (bp) 8616

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-Rpl10a
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1404
  • GenBank ID
    NM_199636.1
  • Entrez Gene
    rpl10a (a.k.a. wu:fb94f08, zgc:73082, zgc:86881)
  • Promoter Takifugu rubripes tyrosine related protein 1 (tyrp)
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer cccaacttccagccttcg
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tol2-ftyrp>TRAP was a gift from Stephen L Johnson (Addgene plasmid # 42299 ; http://n2t.net/addgene:42299 ; RRID:Addgene_42299)

  • For your References section:

    Development of translating ribosome affinity purification for zebrafish. Tryon RC, Pisat N, Johnson SL, Dougherty JD. Genesis. 2012 Dec 19. doi: 10.1002/dvg.22363. 10.1002/dvg.22363 PubMed 23281262
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