pRK5-mWnt8b
(Plasmid
#42285)
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 42285 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRK5
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameWnt8b
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SpeciesM. musculus (mouse)
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Entrez GeneWnt8b
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRK5-mWnt8b was a gift from Chris Garcia & Jeremy Nathans (Addgene plasmid # 42285 ; http://n2t.net/addgene:42285 ; RRID:Addgene_42285) -
For your References section:
Frizzled 2 and frizzled 7 function redundantly in convergent extension and closure of the ventricular septum and palate: evidence for a network of interacting genes. Yu H, Ye X, Guo N, Nathans J. Development. 2012 Dec 1;139(23):4383-94. doi: 10.1242/dev.083352. Epub 2012 Oct 24. 10.1242/dev.083352 PubMed 23095888