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Addgene

pRK5-mFzd2
(Plasmid #42254)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42254 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Frizzled 2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Fzd2 (a.k.a. AL033370, AW456835, Fz10, Fzd10, Mfz10, Mfz10a)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Fzd2 insert contains S193A compared with NM_020510.2. Mutation has no effect on plasmid function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-mFzd2 was a gift from Chris Garcia & Jeremy Nathans (Addgene plasmid # 42254 ; http://n2t.net/addgene:42254 ; RRID:Addgene_42254)

  • For your References section:

    Frizzled 2 and frizzled 7 function redundantly in convergent extension and closure of the ventricular septum and palate: evidence for a network of interacting genes. Yu H, Ye X, Guo N, Nathans J. Development. 2012 Dec 1;139(23):4383-94. doi: 10.1242/dev.083352. Epub 2012 Oct 24. 10.1242/dev.083352 PubMed 23095888
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