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DR274 Citations (193)

Originally described in: Efficient genome editing in zebrafish using a CRISPR-Cas system.
Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT, Yeh JR, Joung JK Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501. PubMed Journal

Articles Citing DR274

Articles
Targeted Heritable Mutation and Gene Conversion by Cas9-CRISPR in Caenorhabditis elegans. Katic I, Grosshans H. Genetics. 2013 Aug 26. PubMed

Associated Plasmids
CRISPR/Cas-mediated targeted mutagenesis in Daphnia magna. Nakanishi T, Kato Y, Matsuura T, Watanabe H. PLoS One. 2014 May 30;9(5):e98363. doi: 10.1371/journal.pone.0098363. eCollection 2014. PubMed
Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Yoshimi K, Kaneko T, Voigt B, Mashimo T. Nat Commun. 2014 Jun 26;5:4240. doi: 10.1038/ncomms5240. PubMed

Associated Plasmids
Landscape of target:guide homology effects on Cas9-mediated cleavage. Fu BX, Hansen LL, Artiles KL, Nonet ML, Fire AZ. Nucleic Acids Res. 2014 Dec 16;42(22):13778-87. doi: 10.1093/nar/gku1102. Epub 2014 Nov 15. PubMed

Associated Plasmids
TALEN and CRISPR/Cas9-mediated genome editing in the early-branching metazoan Nematostella vectensis. Ikmi A, McKinney SA, Delventhal KM, Gibson MC. Nat Commun. 2014 Nov 24;5:5486. doi: 10.1038/ncomms6486. PubMed

Associated Plasmids
High-throughput genome editing and phenotyping facilitated by high resolution melting curve analysis. Thomas HR, Percival SM, Yoder BK, Parant JM. PLoS One. 2014 Dec 11;9(12):e114632. doi: 10.1371/journal.pone.0114632. eCollection 2014. PubMed
A platform for rapid exploration of aging and diseases in a naturally short-lived vertebrate. Harel I, Benayoun BA, Machado B, Singh PP, Hu CK, Pech MF, Valenzano DR, Zhang E, Sharp SC, Artandi SE, Brunet A. Cell. 2015 Feb 26;160(5):1013-26. doi: 10.1016/j.cell.2015.01.038. Epub 2015 Feb 12. PubMed
Generating and identifying axolotls with targeted mutations using Cas9 RNA-guided nuclease. Flowers GP, Crews CM. Methods Mol Biol. 2015;1290:279-95. doi: 10.1007/978-1-4939-2495-0_22. PubMed
Heritable CRISPR/Cas9-mediated genome editing in the yellow fever mosquito, Aedes aegypti. Dong S, Lin J, Held NL, Clem RJ, Passarelli AL, Franz AW. PLoS One. 2015 Mar 27;10(3):e0122353. doi: 10.1371/journal.pone.0122353. eCollection 2015. PubMed
CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes. Liang P, Xu Y, Zhang X, Ding C, Huang R, Zhang Z, Lv J, Xie X, Chen Y, Li Y, Sun Y, Bai Y, Songyang Z, Ma W, Zhou C, Huang J. Protein Cell. 2015 May;6(5):363-72. doi: 10.1007/s13238-015-0153-5. Epub 2015 Apr 18. PubMed
CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool. Stemmer M, Thumberger T, Del Sol Keyer M, Wittbrodt J, Mateo JL. PLoS One. 2015 Apr 24;10(4):e0124633. doi: 10.1371/journal.pone.0124633. eCollection 2015. PubMed
Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafish. Ogawa Y, Shiraki T, Kojima D, Fukada Y. Proc Biol Sci. 2015 Aug 7;282(1812). pii: 20150659. doi: 10.1098/rspb.2015.0659. PubMed
Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins. Menoret S, De Cian A, Tesson L, Remy S, Usal C, Boule JB, Boix C, Fontaniere S, Creneguy A, Nguyen TH, Brusselle L, Thinard R, Gauguier D, Concordet JP, Cherifi Y, Fraichard A, Giovannangeli C, Anegon I. Sci Rep. 2015 Oct 7;5:14410. doi: 10.1038/srep14410. PubMed
The alternative splicing factor Nova2 regulates vascular development and lumen formation. Giampietro C, Deflorian G, Gallo S, Di Matteo A, Pradella D, Bonomi S, Belloni E, Nyqvist D, Quaranta V, Confalonieri S, Bertalot G, Orsenigo F, Pisati F, Ferrero E, Biamonti G, Fredrickx E, Taveggia C, Wyatt CD, Irimia M, Di Fiore PP, Blencowe BJ, Dejana E, Ghigna C. Nat Commun. 2015 Oct 8;6:8479. doi: 10.1038/ncomms9479. PubMed
An autoregulatory enhancer controls mammary-specific STAT5 functions. Metser G, Shin HY, Wang C, Yoo KH, Oh S, Villarino AV, O'Shea JJ, Kang K, Hennighausen L. Nucleic Acids Res. 2015 Oct 7. pii: gkv999. PubMed
An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish. Zou J, Tran D, Baalbaki M, Tang LF, Poon A, Pelonero A, Titus EW, Yuan C, Shi C, Patchava S, Halper E, Garg J, Movsesyan I, Yin C, Wu R, Wilsbacher LD, Liu J, Hager RL, Coughlin SR, Jinek M, Pullinger CR, Kane JP, Hart DO, Kwok PY, Deo RC. Elife. 2015 Oct 16;4. pii: e09406. doi: 10.7554/eLife.09406. PubMed
A Zebrafish Loss-of-Function Model for Human CFAP53 Mutations Reveals Its Specific Role in Laterality Organ Function. Noel ES, Momenah TS, Al-Dagriri K, Al-Suwaid A, Al-Shahrani S, Jiang H, Willekers S, Oostveen YY, Chocron S, Postma AV, Bhuiyan ZA, Bakkers J. Hum Mutat. 2016 Feb;37(2):194-200. doi: 10.1002/humu.22928. Epub 2015 Dec 2. PubMed
CRISPR/Cas9: An inexpensive, efficient loss of function tool to screen human disease genes in Xenopus. Bhattacharya D, Marfo CA, Li D, Lane M, Khokha MK. Dev Biol. 2015 Dec 15;408(2):196-204. doi: 10.1016/j.ydbio.2015.11.003. Epub 2015 Nov 4. PubMed
CRISPR/Cas9 Promotes Functional Study of Testis Specific X-Linked Gene In Vivo. Li M, Huang R, Jiang X, Chen Y, Zhang Z, Zhang X, Liang P, Zhan S, Cao S, Songyang Z, Huang J. PLoS One. 2015 Nov 24;10(11):e0143148. doi: 10.1371/journal.pone.0143148. eCollection 2015. PubMed
TALEN-mediated homologous recombination in Daphnia magna. Nakanishi T, Kato Y, Matsuura T, Watanabe H. Sci Rep. 2015 Dec 17;5:18312. doi: 10.1038/srep18312. PubMed
A possible aid in targeted insertion of large DNA elements by CRISPR/Cas in mouse zygotes. Nakao H, Harada T, Nakao K, Kiyonari H, Inoue K, Furuta Y, Aiba A. Genesis. 2015 Dec 29. doi: 10.1002/dvg.22914. PubMed
ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Yoshimi K, Kunihiro Y, Kaneko T, Nagahora H, Voigt B, Mashimo T. Nat Commun. 2016 Jan 20;7:10431. doi: 10.1038/ncomms10431. PubMed

Associated Plasmids
A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs. Romanienko PJ, Giacalone J, Ingenito J, Wang Y, Isaka M, Johnson T, You Y, Mark WH. PLoS One. 2016 Feb 5;11(2):e0148362. doi: 10.1371/journal.pone.0148362. eCollection 2016. PubMed

Associated Plasmids
Stereocilia-staircase spacing is influenced by myosin III motors and their cargos espin-1 and espin-like. Ebrahim S, Avenarius MR, Grati M, Krey JF, Windsor AM, Sousa AD, Ballesteros A, Cui R, Millis BA, Salles FT, Baird MA, Davidson MW, Jones SM, Choi D, Dong L, Raval MH, Yengo CM, Barr-Gillespie PG, Kachar B. Nat Commun. 2016 Mar 1;7:10833. doi: 10.1038/ncomms10833. PubMed
2C-Cas9: a versatile tool for clonal analysis of gene function. Di Donato V, De Santis F, Auer TO, Testa N, Sanchez-Iranzo H, Mercader N, Concordet JP, Del Bene F. Genome Res. 2016 Mar 8. PubMed

Associated Plasmids
Epilepsy, Behavioral Abnormalities, and Physiological Comorbidities in Syntaxin-Binding Protein 1 (STXBP1) Mutant Zebrafish. Grone BP, Marchese M, Hamling KR, Kumar MG, Krasniak CS, Sicca F, Santorelli FM, Patel M, Baraban SC. PLoS One. 2016 Mar 10;11(3):e0151148. doi: 10.1371/journal.pone.0151148. eCollection 2016. PubMed
Efficient genome editing of genes involved in neural crest development using the CRISPR/Cas9 system in Xenopus embryos. Liu Z, Cheng TT, Shi Z, Liu Z, Lei Y, Wang C, Shi W, Chen X, Qi X, Cai D, Feng B, Deng Y, Chen Y, Zhao H. Cell Biosci. 2016 Mar 31;6:22. doi: 10.1186/s13578-016-0088-4. eCollection 2016. PubMed
ADAM12-deficient zebrafish exhibit retardation in body growth at the juvenile stage without developmental defects. Tokumasu Y, Iida A, Wang Z, Ansai S, Kinoshita M, Sehara-Fujisawa A. Dev Growth Differ. 2016 May;58(4):409-21. doi: 10.1111/dgd.12286. Epub 2016 May 16. PubMed
tfec controls the hematopoietic stem cell vascular niche during zebrafish embryogenesis. Mahony CB, Fish RJ, Pasche C, Bertrand JY. Blood. 2016 Sep 8;128(10):1336-45. doi: 10.1182/blood-2016-04-710137. Epub 2016 Jul 11. PubMed
Homology-Independent Integration of Plasmid DNA into the Zebrafish Genome. Auer TO, Del Bene F. Methods Mol Biol. 2016;1451:31-51. doi: 10.1007/978-1-4939-3771-4_3. PubMed
Detection of Multiple Genome Modifications Induced by the CRISPR/Cas9 System. Ota S, Kawahara A. Methods Mol Biol. 2016;1451:53-63. doi: 10.1007/978-1-4939-3771-4_4. PubMed
Electroporation of Cas9 protein/sgRNA into early pronuclear zygotes generates non-mosaic mutants in the mouse. Hashimoto M, Yamashita Y, Takemoto T. Dev Biol. 2016 Oct 1;418(1):1-9. doi: 10.1016/j.ydbio.2016.07.017. Epub 2016 Jul 26. PubMed
Dephosphorylated parafibromin is a transcriptional coactivator of the Wnt/Hedgehog/Notch pathways. Kikuchi I, Takahashi-Kanemitsu A, Sakiyama N, Tang C, Tang PJ, Noda S, Nakao K, Kassai H, Sato T, Aiba A, Hatakeyama M. Nat Commun. 2016 Sep 21;7:12887. doi: 10.1038/ncomms12887. PubMed
Deep Brain Photoreceptor (val-opsin) Gene Knockout Using CRISPR/Cas Affects Chorion Formation and Embryonic Hatching in the Zebrafish. Hang CY, Moriya S, Ogawa S, Parhar IS. PLoS One. 2016 Oct 28;11(10):e0165535. doi: 10.1371/journal.pone.0165535. eCollection 2016. PubMed
Knockout of Zebrafish Ovarian Aromatase Gene (cyp19a1a) by TALEN and CRISPR/Cas9 Leads to All-male Offspring Due to Failed Ovarian Differentiation. Lau ES, Zhang Z, Qin M, Ge W. Sci Rep. 2016 Nov 23;6:37357. doi: 10.1038/srep37357. PubMed
Neuronal Ndrg4 Is Essential for Nodes of Ranvier Organization in Zebrafish. Fontenas L, De Santis F, Di Donato V, Degerny C, Chambraud B, Del Bene F, Tawk M. PLoS Genet. 2016 Nov 30;12(11):e1006459. doi: 10.1371/journal.pgen.1006459. eCollection 2016 Nov. PubMed
A vertebrate-specific and essential role for osterix in osteogenesis revealed by gene knockout in the teleost medaka. Yu T, Graf M, Renn J, Schartl M, Larionova D, Huysseune A, Witten PE, Winkler C. Development. 2017 Jan 15;144(2):265-271. doi: 10.1242/dev.139550. Epub 2016 Dec 19. PubMed
Lineage-Specific and Non-specific Cytokine-Sensing Genes Respond Differentially to the Master Regulator STAT5. Zeng X, Willi M, Shin HY, Hennighausen L, Wang C. Cell Rep. 2016 Dec 20;17(12):3333-3346. doi: 10.1016/j.celrep.2016.11.079. PubMed
Neuronal sFlt1 and Vegfaa determine venous sprouting and spinal cord vascularization. Wild R, Klems A, Takamiya M, Hayashi Y, Strahle U, Ando K, Mochizuki N, van Impel A, Schulte-Merker S, Krueger J, Preau L, le Noble F. Nat Commun. 2017 Jan 10;8:13991. doi: 10.1038/ncomms13991. PubMed
Sequential organogenesis sets two parallel sensory lines in medaka. Seleit A, Kramer I, Ambrosio E, Dross N, Engel U, Centanin L. Development. 2017 Feb 15;144(4):687-697. doi: 10.1242/dev.142752. Epub 2017 Jan 13. PubMed
Expression of the novel maternal centrosome assembly factor Wdr8 is required for vertebrate embryonic mitoses. Inoue D, Stemmer M, Thumberger T, Ruppert T, Barenz F, Wittbrodt J, Gruss OJ. Nat Commun. 2017 Jan 18;8:14090. doi: 10.1038/ncomms14090. PubMed
A Unique Egg Cortical Granule Localization Motif Is Required for Ovastacin Sequestration to Prevent Premature ZP2 Cleavage and Ensure Female Fertility in Mice. Xiong B, Zhao Y, Beall S, Sadusky AB, Dean J. PLoS Genet. 2017 Jan 23;13(1):e1006580. doi: 10.1371/journal.pgen.1006580. eCollection 2017 Jan. PubMed
Protanopia (red color-blindness) in medaka: a simple system for producing color-blind fish and testing their spectral sensitivity. Homma N, Harada Y, Uchikawa T, Kamei Y, Fukamachi S. BMC Genet. 2017 Feb 6;18(1):10. doi: 10.1186/s12863-017-0477-7. PubMed
CRISPR-Mediated Knockout of Cybb in NSG Mice Establishes a Model of Chronic Granulomatous Disease for Human Stem-Cell Gene Therapy Transplants. Sweeney CL, Choi U, Liu C, Koontz S, Ha SK, Malech HL. Hum Gene Ther. 2017 Jul;28(7):565-575. doi: 10.1089/hum.2017.005. Epub 2017 Mar 6. PubMed
ORAI2 modulates store-operated calcium entry and T cell-mediated immunity. Vaeth M, Yang J, Yamashita M, Zee I, Eckstein M, Knosp C, Kaufmann U, Karoly Jani P, Lacruz RS, Flockerzi V, Kacskovics I, Prakriya M, Feske S. Nat Commun. 2017 Mar 15;8:14714. doi: 10.1038/ncomms14714. PubMed
Co-accumulation of cis-regulatory and coding mutations during the pseudogenization of the Xenopus laevis homoeologs six6.L and six6.S. Ochi H, Kawaguchi A, Tanouchi M, Suzuki N, Kumada T, Iwata Y, Ogino H. Dev Biol. 2017 Jul 1;427(1):84-92. doi: 10.1016/j.ydbio.2017.05.004. Epub 2017 May 10. PubMed
CRISPR/Cas9 targeting events cause complex deletions and insertions at 17 sites in the mouse genome. Shin HY, Wang C, Lee HK, Yoo KH, Zeng X, Kuhns T, Yang CM, Mohr T, Liu C, Hennighausen L. Nat Commun. 2017 May 31;8:15464. doi: 10.1038/ncomms15464. PubMed
CRISPR/Cas9-Mediated Targeted Knockin of Exogenous Reporter Genes in Zebrafish. Kawahara A. Methods Mol Biol. 2017;1630:165-173. doi: 10.1007/978-1-4939-7128-2_14. PubMed
Genome Editing of Medaka. Ansai S, Kinoshita M. Methods Mol Biol. 2017;1630:175-188. doi: 10.1007/978-1-4939-7128-2_15. PubMed
A novel humanized mouse lacking murine P450 oxidoreductase for studying human drug metabolism. Barzi M, Pankowicz FP, Zorman B, Liu X, Legras X, Yang D, Borowiak M, Bissig-Choisat B, Sumazin P, Li F, Bissig KD. Nat Commun. 2017 Jun 28;8(1):39. doi: 10.1038/s41467-017-00049-x. PubMed
CRISPR/Cas9-Directed Gene Editing for the Generation of Loss-of-Function Mutants in High-Throughput Zebrafish F0 Screens. Shankaran SS, Dahlem TJ, Bisgrove BW, Yost HJ, Tristani-Firouzi M. Curr Protoc Mol Biol. 2017 Jul 5;119:31.9.1-31.9.22. doi: 10.1002/cpmb.42. PubMed
An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes). Murakami Y, Ansai S, Yonemura A, Kinoshita M. Zoological Lett. 2017 Jul 6;3:10. doi: 10.1186/s40851-017-0071-x. eCollection 2017. PubMed
Dynamics of in vivo ASC speck formation. Kuri P, Schieber NL, Thumberger T, Wittbrodt J, Schwab Y, Leptin M. J Cell Biol. 2017 Sep 4;216(9):2891-2909. doi: 10.1083/jcb.201703103. Epub 2017 Jul 12. PubMed
Modeling Dominant and Recessive Forms of Retinitis Pigmentosa by Editing Three Rhodopsin-Encoding Genes in Xenopus Laevis Using Crispr/Cas9. Feehan JM, Chiu CN, Stanar P, Tam BM, Ahmed SN, Moritz OL. Sci Rep. 2017 Jul 31;7(1):6920. doi: 10.1038/s41598-017-07153-4. PubMed
Zygotic vinculin is not essential for embryonic development in zebrafish. Han MKL, van der Krogt GNM, de Rooij J. PLoS One. 2017 Aug 2;12(8):e0182278. doi: 10.1371/journal.pone.0182278. eCollection 2017. PubMed
Fibroblast growth factor signaling is required for early somatic gonad development in zebrafish. Leerberg DM, Sano K, Draper BW. PLoS Genet. 2017 Sep 5;13(9):e1006993. doi: 10.1371/journal.pgen.1006993. eCollection 2017 Sep. PubMed
A right-handed signalling pathway drives heart looping in vertebrates. Ocana OH, Coskun H, Minguillon C, Murawala P, Tanaka EM, Galceran J, Munoz-Chapuli R, Nieto MA. Nature. 2017 Sep 6;549(7670):86-90. doi: 10.1038/nature23454. PubMed
Using CRISPR/Cas9-mediated gene editing to further explore growth and trade-off effects in myostatin-mutated F4 medaka (Oryzias latipes). Yeh YC, Kinoshita M, Ng TH, Chang YH, Maekawa S, Chiang YA, Aoki T, Wang HC. Sci Rep. 2017 Sep 12;7(1):11435. doi: 10.1038/s41598-017-09966-9. PubMed
Mutations of conserved non-coding elements of PITX2 in patients with ocular dysgenesis and developmental glaucoma. Protas ME, Weh E, Footz T, Kasberger J, Baraban SC, Levin AV, Katz LJ, Ritch R, Walter MA, Semina EV, Gould DB. Hum Mol Genet. 2017 Sep 15;26(18):3630-3638. doi: 10.1093/hmg/ddx251. PubMed
Correction of beta-thalassemia mutant by base editor in human embryos. Liang P, Ding C, Sun H, Xie X, Xu Y, Zhang X, Sun Y, Xiong Y, Ma W, Liu Y, Wang Y, Fang J, Liu D, Songyang Z, Zhou C, Huang J. Protein Cell. 2017 Nov;8(11):811-822. doi: 10.1007/s13238-017-0475-6. Epub 2017 Sep 23. PubMed
Combined CRISPRi/a-Based Chemical Genetic Screens Reveal that Rigosertib Is a Microtubule-Destabilizing Agent. Jost M, Chen Y, Gilbert LA, Horlbeck MA, Krenning L, Menchon G, Rai A, Cho MY, Stern JJ, Prota AE, Kampmann M, Akhmanova A, Steinmetz MO, Tanenbaum ME, Weissman JS. Mol Cell. 2017 Oct 5;68(1):210-223.e6. doi: 10.1016/j.molcel.2017.09.012. PubMed
Pyridoxine-Dependent Epilepsy in Zebrafish Caused by Aldh7a1 Deficiency. Pena IA, Roussel Y, Daniel K, Mongeon K, Johnstone D, Weinschutz Mendes H, Bosma M, Saxena V, Lepage N, Chakraborty P, Dyment DA, van Karnebeek CDM, Verhoeven-Duif N, Bui TV, Boycott KM, Ekker M, MacKenzie A. Genetics. 2017 Dec;207(4):1501-1518. doi: 10.1534/genetics.117.300137. Epub 2017 Oct 23. PubMed
Efficient gene knockin in axolotl and its use to test the role of satellite cells in limb regeneration. Fei JF, Schuez M, Knapp D, Taniguchi Y, Drechsel DN, Tanaka EM. Proc Natl Acad Sci U S A. 2017 Nov 21;114(47):12501-12506. doi: 10.1073/pnas.1706855114. Epub 2017 Oct 31. PubMed

Associated Plasmids
An Intracellular Pathogen Response Pathway Promotes Proteostasis in C. elegans. Reddy KC, Dror T, Sowa JN, Panek J, Chen K, Lim ES, Wang D, Troemel ER. Curr Biol. 2017 Nov 20;27(22):3544-3553.e5. doi: 10.1016/j.cub.2017.10.009. Epub 2017 Nov 2. PubMed
KIAA1109 Variants Are Associated with a Severe Disorder of Brain Development and Arthrogryposis. Gueneau L, Fish RJ, Shamseldin HE, Voisin N, Tran Mau-Them F, Preiksaitiene E, Monroe GR, Lai A, Putoux A, Allias F, Ambusaidi Q, Ambrozaityte L, Cimbalistiene L, Delafontaine J, Guex N, Hashem M, Kurdi W, Jamuar SS, Ying LJ, Bonnard C, Pippucci T, Pradervand S, Roechert B, van Hasselt PM, Wiederkehr M, Wright CF, Xenarios I, van Haaften G, Shaw-Smith C, Schindewolf EM, Neerman-Arbez M, Sanlaville D, Lesca G, Guibaud L, Reversade B, Chelly J, Kucinskas V, Alkuraya FS, Reymond A. Am J Hum Genet. 2018 Jan 4;102(1):116-132. doi: 10.1016/j.ajhg.2017.12.002. Epub 2017 Dec 28. PubMed
A gonad-expressed opsin mediates light-induced spawning in the jellyfish Clytia. Quiroga Artigas G, Lapebie P, Leclere L, Takeda N, Deguchi R, Jekely G, Momose T, Houliston E. Elife. 2018 Jan 5;7. doi: 10.7554/eLife.29555. PubMed
Knockdown of Laminin gamma-3 (Lamc3) impairs motoneuron guidance in the zebrafish embryo. Eve AMJ, Smith JC. Wellcome Open Res. 2017 Nov 16;2:111. doi: 10.12688/wellcomeopenres.12394.1. eCollection 2017. PubMed
An Attractive Reelin Gradient Establishes Synaptic Lamination in the Vertebrate Visual System. Di Donato V, De Santis F, Albadri S, Auer TO, Duroure K, Charpentier M, Concordet JP, Gebhardt C, Del Bene F. Neuron. 2018 Mar 7;97(5):1049-1062.e6. doi: 10.1016/j.neuron.2018.01.030. Epub 2018 Feb 8. PubMed
Germ cells in the teleost fish medaka have an inherent feminizing effect. Nishimura T, Yamada K, Fujimori C, Kikuchi M, Kawasaki T, Siegfried KR, Sakai N, Tanaka M. PLoS Genet. 2018 Mar 29;14(3):e1007259. doi: 10.1371/journal.pgen.1007259. eCollection 2018 Mar. PubMed
Identification of an Intra- and Inter-specific Tear Protein Signal in Rodents. Tsunoda M, Miyamichi K, Eguchi R, Sakuma Y, Yoshihara Y, Kikusui T, Kuwahara M, Touhara K. Curr Biol. 2018 Apr 23;28(8):1213-1223.e6. doi: 10.1016/j.cub.2018.02.060. Epub 2018 Mar 29. PubMed
A Forward Genetic Screen in Zebrafish Identifies the G-Protein-Coupled Receptor CaSR as a Modulator of Sensorimotor Decision Making. Jain RA, Wolman MA, Marsden KC, Nelson JC, Shoenhard H, Echeverry FA, Szi C, Bell H, Skinner J, Cobbs EN, Sawada K, Zamora AD, Pereda AE, Granato M. Curr Biol. 2018 May 7;28(9):1357-1369.e5. doi: 10.1016/j.cub.2018.03.025. Epub 2018 Apr 19. PubMed
Efficient Gene Transfer and Gene Editing in Sterlet (Acipenser ruthenus). Chen J, Wang W, Tian Z, Dong Y, Dong T, Zhu H, Zhu Z, Hu H, Hu W. Front Genet. 2018 Apr 6;9:117. doi: 10.3389/fgene.2018.00117. eCollection 2018. PubMed
Small GTPase R-Ras participates in neural tube formation in zebrafish embryonic spinal cord. Ohata S, Uga H, Okamoto H, Katada T. Biochem Biophys Res Commun. 2018 Jun 27;501(3):786-790. doi: 10.1016/j.bbrc.2018.05.074. Epub 2018 May 18. PubMed
Usherin defects lead to early-onset retinal dysfunction in zebrafish. Dona M, Slijkerman R, Lerner K, Broekman S, Wegner J, Howat T, Peters T, Hetterschijt L, Boon N, de Vrieze E, Sorusch N, Wolfrum U, Kremer H, Neuhauss S, Zang J, Kamermans M, Westerfield M, Phillips J, van Wijk E. Exp Eye Res. 2018 Aug;173:148-159. doi: 10.1016/j.exer.2018.05.015. Epub 2018 May 16. PubMed
rbpms2 functions in Balbiani body architecture and ovary fate. Kaufman OH, Lee K, Martin M, Rothhamel S, Marlow FL. PLoS Genet. 2018 Jul 5;14(7):e1007489. doi: 10.1371/journal.pgen.1007489. eCollection 2018 Jul. PubMed
Genome editing of pufferfish saxitoxin- and tetrodotoxin-binding protein type 2 in Takifugu rubripes. Kato-Unoki Y, Takai Y, Kinoshita M, Mochizuki T, Tatsuno R, Shimasaki Y, Oshima Y. Toxicon. 2018 Oct;153:58-61. doi: 10.1016/j.toxicon.2018.08.001. Epub 2018 Aug 29. PubMed
MiR-202 controls female fecundity by regulating medaka oogenesis. Gay S, Bugeon J, Bouchareb A, Henry L, Delahaye C, Legeai F, Montfort J, Le Cam A, Siegel A, Bobe J, Thermes V. PLoS Genet. 2018 Sep 10;14(9):e1007593. doi: 10.1371/journal.pgen.1007593. eCollection 2018 Sep. PubMed
Sequential specification of oligodendrocyte lineage cells by distinct levels of Hedgehog and Notch signaling. Ravanelli AM, Kearns CA, Powers RK, Wang Y, Hines JH, Donaldson MJ, Appel B. Dev Biol. 2018 Dec 15;444(2):93-106. doi: 10.1016/j.ydbio.2018.10.004. Epub 2018 Oct 19. PubMed
Using CRISPR/Cas9 for Gene Knockout in Immunodeficient NSG Mice. Du Y, Xie W, Zhang F, Choi U, Liu C, Sweeney CL. Methods Mol Biol. 2019;1874:139-168. doi: 10.1007/978-1-4939-8831-0_8. PubMed
Sexual rejection via a vomeronasal receptor-triggered limbic circuit. Osakada T, Ishii KK, Mori H, Eguchi R, Ferrero DM, Yoshihara Y, Liberles SD, Miyamichi K, Touhara K. Nat Commun. 2018 Oct 26;9(1):4463. doi: 10.1038/s41467-018-07003-5. PubMed
Generation of white-eyed Daphnia magna mutants lacking scarlet function. Ismail NIB, Kato Y, Matsuura T, Watanabe H. PLoS One. 2018 Nov 14;13(11):e0205609. doi: 10.1371/journal.pone.0205609. eCollection 2018. PubMed
Application and optimization of CRISPR-Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum). Fei JF, Lou WP, Knapp D, Murawala P, Gerber T, Taniguchi Y, Nowoshilow S, Khattak S, Tanaka EM. Nat Protoc. 2018 Dec;13(12):2908-2943. doi: 10.1038/s41596-018-0071-0. PubMed
A CRISPR-Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection. Zhou W, Hu L, Ying L, Zhao Z, Chu PK, Yu XF. Nat Commun. 2018 Nov 27;9(1):5012. doi: 10.1038/s41467-018-07324-5. PubMed
Off- and on-target effects of genome editing in mouse embryos. Ayabe S, Nakashima K, Yoshiki A. J Reprod Dev. 2019 Feb 8;65(1):1-5. doi: 10.1262/jrd.2018-128. Epub 2018 Dec 6. PubMed
Neural circuitry of a polycystin-mediated hydrodynamic startle response for predator avoidance. Bezares-Calderon LA, Berger J, Jasek S, Veraszto C, Mendes S, Guhmann M, Almeda R, Shahidi R, Jekely G. Elife. 2018 Dec 14;7. pii: 36262. doi: 10.7554/eLife.36262. PubMed
Ciliary Beating Compartmentalizes Cerebrospinal Fluid Flow in the Brain and Regulates Ventricular Development. Olstad EW, Ringers C, Hansen JN, Wens A, Brandt C, Wachten D, Yaksi E, Jurisch-Yaksi N. Curr Biol. 2019 Jan 21;29(2):229-241.e6. doi: 10.1016/j.cub.2018.11.059. Epub 2019 Jan 3. PubMed
Simultaneous targeting of linked loci in mouse embryos using base editing. Lee HK, Willi M, Smith HE, Miller SM, Liu DR, Liu C, Hennighausen L. Sci Rep. 2019 Feb 7;9(1):1662. doi: 10.1038/s41598-018-33533-5. PubMed
Nuclear Progestin Receptor Phosphorylation by Cdk9 Is Required for the Expression of Mmp15, a Protease Indispensable for Ovulation in Medaka. Ogiwara K, Takahashi T. Cells. 2019 Mar 4;8(3). pii: cells8030215. doi: 10.3390/cells8030215. PubMed
Enhanced in vivo-imaging in medaka by optimized anaesthesia, fluorescent protein selection and removal of pigmentation. Lischik CQ, Adelmann L, Wittbrodt J. PLoS One. 2019 Mar 7;14(3):e0212956. doi: 10.1371/journal.pone.0212956. eCollection 2019. PubMed
LAMP5 in presynaptic inhibitory terminals in the hindbrain and spinal cord: a role in startle response and auditory processing. Koebis M, Urata S, Shinoda Y, Okabe S, Yamasoba T, Nakao K, Aiba A, Furuichi T. Mol Brain. 2019 Mar 12;12(1):20. doi: 10.1186/s13041-019-0437-4. PubMed
Targeted in vivo epigenome editing of H3K27me3. Fukushima HS, Takeda H, Nakamura R. Epigenetics Chromatin. 2019 Mar 13;12(1):17. doi: 10.1186/s13072-019-0263-z. PubMed

Associated Plasmids
The central nervous system acts as a transducer of stress-induced masculinization through corticotropin-releasing hormone B. Castaneda Cortes DC, Padilla LFA, Langlois VS, Somoza GM, Fernandino JI. Development. 2019 Apr 23;146(8). pii: dev.172866. doi: 10.1242/dev.172866. PubMed
Genomic insights into HSFs as candidate genes for high-temperature stress adaptation and gene editing with minimal off-target effects in flax. Saha D, Mukherjee P, Dutta S, Meena K, Sarkar SK, Mandal AB, Dasgupta T, Mitra J. Sci Rep. 2019 Apr 3;9(1):5581. doi: 10.1038/s41598-019-41936-1. PubMed
A novel evolutionary conserved mechanism of RNA stability regulates synexpression of primordial germ cell-specific genes prior to the sex-determination stage in medaka. Herpin A, Schmidt C, Kneitz S, Gobe C, Regensburger M, Le Cam A, Montfort J, Adolfi MC, Lillesaar C, Wilhelm D, Kraeussling M, Mourot B, Porcon B, Pannetier M, Pailhoux E, Ettwiller L, Dolle D, Guiguen Y, Schartl M. PLoS Biol. 2019 Apr 4;17(4):e3000185. doi: 10.1371/journal.pbio.3000185. eCollection 2019 Apr. PubMed
Loss of atrx cooperates with p53-deficiency to promote the development of sarcomas and other malignancies. Oppel F, Tao T, Shi H, Ross KN, Zimmerman MW, He S, Tong G, Aster JC, Look AT. PLoS Genet. 2019 Apr 10;15(4):e1008039. doi: 10.1371/journal.pgen.1008039. eCollection 2019 Apr. PubMed
Autophagy in Xenopus laevis rod photoreceptors is independently regulated by phototransduction and misfolded RHO(P23H). Wen RH, Stanar P, Tam B, Moritz OL. Autophagy. 2019 Apr 12:1-20. doi: 10.1080/15548627.2019.1596487. PubMed
Mouse ANKRD31 Regulates Spatiotemporal Patterning of Meiotic Recombination Initiation and Ensures Recombination between X and Y Sex Chromosomes. Papanikos F, Clement JAJ, Testa E, Ravindranathan R, Grey C, Dereli I, Bondarieva A, Valerio-Cabrera S, Stanzione M, Schleiffer A, Jansa P, Lustyk D, Fei JF, Adams IR, Forejt J, Barchi M, de Massy B, Toth A. Mol Cell. 2019 Jun 6;74(5):1069-1085.e11. doi: 10.1016/j.molcel.2019.03.022. Epub 2019 Apr 15. PubMed
An integrated protocol for targeted mutagenesis with CRISPR-Cas9 system in the pea aphid. Le Trionnaire G, Tanguy S, Hudaverdian S, Gleonnec F, Richard G, Cayrol B, Monsion B, Pichon E, Deshoux M, Webster C, Uzest M, Herpin A, Tagu D. Insect Biochem Mol Biol. 2019 Jul;110:34-44. doi: 10.1016/j.ibmb.2019.04.016. Epub 2019 Apr 20. PubMed
Fgf3 is crucial for the generation of monoaminergic cerebrospinal fluid contacting cells in zebrafish. Reuter I, Jackels J, Kneitz S, Kuper J, Lesch KP, Lillesaar C. Biol Open. 2019 Jun 5;8(6). pii: bio.040683. doi: 10.1242/bio.040683. PubMed
yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets. Vazquez-Marin J, Gutierrez-Triana JA, Almuedo-Castillo M, Buono L, Gomez-Skarmeta JL, Mateo JL, Wittbrodt J, Martinez-Morales JR. Development. 2019 Jun 21;146(13). pii: dev.173286. doi: 10.1242/dev.173286. PubMed
Elmo1 function, linked to Rac1 activity, regulates peripheral neuronal numbers and myelination in zebrafish. Mikdache A, Fontenas L, Albadri S, Revenu C, Loisel-Duwattez J, Lesport E, Degerny C, Del Bene F, Tawk M. Cell Mol Life Sci. 2020 Jan;77(1):161-177. doi: 10.1007/s00018-019-03167-5. Epub 2019 Jun 3. PubMed
Fibroblast Growth Factor Receptors Function Redundantly During Zebrafish Embryonic Development. Leerberg DM, Hopton RE, Draper BW. Genetics. 2019 Jun 7. pii: genetics.119.302345. doi: 10.1534/genetics.119.302345. PubMed
TRAF6 function as a novel co-regulator of Wnt3a target genes in prostate cancer. Aripaka K, Gudey SK, Zang G, Schmidt A, Ahrling SS, Osterman L, Bergh A, von Hofsten J, Landstrom M. EBioMedicine. 2019 Jul;45:192-207. doi: 10.1016/j.ebiom.2019.06.046. Epub 2019 Jun 28. PubMed
Precise Short Sequence Insertion in Zebrafish Using a CRISPR/Cas9 Approach to Generate a Constitutively Soluble Lrp2 Protein. Collery RF, Link BA. Front Cell Dev Biol. 2019 Aug 13;7:167. doi: 10.3389/fcell.2019.00167. eCollection 2019. PubMed
Actomyosin regulation by Eph receptor signaling couples boundary cell formation to border sharpness. Cayuso J, Xu Q, Addison M, Wilkinson DG. Elife. 2019 Sep 10;8. pii: 49696. doi: 10.7554/eLife.49696. PubMed
TEADs, Yap, Taz, Vgll4s transcription factors control the establishment of Left-Right asymmetry in zebrafish. Fillatre J, Fauny JD, Fels JA, Li C, Goll M, Thisse C, Thisse B. Elife. 2019 Sep 12;8. pii: 45241. doi: 10.7554/eLife.45241. PubMed
ECM alterations in Fndc3a (Fibronectin Domain Containing Protein 3A) deficient zebrafish cause temporal fin development and regeneration defects. Liedtke D, Orth M, Meissler M, Geuer S, Knaup S, Koblitz I, Klopocki E. Sci Rep. 2019 Sep 16;9(1):13383. doi: 10.1038/s41598-019-50055-w. PubMed
Valves Are a Conserved Feature of the Zebrafish Lymphatic System. Shin M, Nozaki T, Idrizi F, Isogai S, Ogasawara K, Ishida K, Yuge S, Roscoe B, Wolfe SA, Fukuhara S, Mochizuki N, Deguchi T, Lawson ND. Dev Cell. 2019 Nov 4;51(3):374-386.e5. doi: 10.1016/j.devcel.2019.08.019. Epub 2019 Sep 26. PubMed

Associated Plasmids
Sertoli cell-only phenotype and scRNA-seq define PRAMEF12 as a factor essential for spermatogenesis in mice. Wang Z, Xu X, Li JL, Palmer C, Maric D, Dean J. Nat Commun. 2019 Nov 15;10(1):5196. doi: 10.1038/s41467-019-13193-3. PubMed
A morphogenetic EphB/EphrinB code controls hepatopancreatic duct formation. Thestrup MI, Caviglia S, Cayuso J, Heyne RLS, Ahmad R, Hofmeister W, Satriano L, Wilkinson DG, Andersen JB, Ober EA. Nat Commun. 2019 Nov 19;10(1):5220. doi: 10.1038/s41467-019-13149-7. PubMed
Galanin Signaling in the Brain Regulates Color Pattern Formation in Zebrafish. Eskova A, Frohnhofer HG, Nusslein-Volhard C, Irion U. Curr Biol. 2020 Jan 20;30(2):298-303.e3. doi: 10.1016/j.cub.2019.11.033. Epub 2020 Jan 2. PubMed
Origin and role of the cerebrospinal fluid bidirectional flow in the central canal. Thouvenin O, Keiser L, Cantaut-Belarif Y, Carbo-Tano M, Verweij F, Jurisch-Yaksi N, Bardet PL, van Niel G, Gallaire F, Wyart C. Elife. 2020 Jan 9;9. pii: 47699. doi: 10.7554/eLife.47699. PubMed
Translation elongation factor 1A2 is encoded by one of four closely related eef1a genes and is dispensable for survival in zebrafish. Idigo NJ, Soares DC, Abbott CM. Biosci Rep. 2020 Jan 31;40(1). pii: 221899. doi: 10.1042/BSR20194191. PubMed
Effective generation of maternal genome point mutated porcine embryos by injection of cytosine base editor into germinal vesicle oocytes. Su X, Chen W, Cai Q, Liang P, Chen Y, Cong P, Huang J. Sci China Life Sci. 2020 Jul;63(7):996-1005. doi: 10.1007/s11427-019-1611-1. Epub 2020 Jan 21. PubMed
Systemic loss of Sarm1 protects Schwann cells from chemotoxicity by delaying axon degeneration. Tian W, Czopka T, Lopez-Schier H. Commun Biol. 2020 Jan 30;3(1):49. doi: 10.1038/s42003-020-0776-9. PubMed
FIGLA, LHX8 and SOHLH1 transcription factor networks regulate mouse oocyte growth and differentiation. Wang Z, Liu CY, Zhao Y, Dean J. Nucleic Acids Res. 2020 Apr 17;48(7):3525-3541. doi: 10.1093/nar/gkaa101. PubMed
Pathogenic POGZ mutation causes impaired cortical development and reversible autism-like phenotypes. Matsumura K, Seiriki K, Okada S, Nagase M, Ayabe S, Yamada I, Furuse T, Shibuya H, Yasuda Y, Yamamori H, Fujimoto M, Nagayasu K, Yamamoto K, Kitagawa K, Miura H, Gotoda-Nishimura N, Igarashi H, Hayashida M, Baba M, Kondo M, Hasebe S, Ueshima K, Kasai A, Ago Y, Hayata-Takano A, Shintani N, Iguchi T, Sato M, Yamaguchi S, Tamura M, Wakana S, Yoshiki A, Watabe AM, Okano H, Takuma K, Hashimoto R, Hashimoto H, Nakazawa T. Nat Commun. 2020 Feb 26;11(1):859. doi: 10.1038/s41467-020-14697-z. PubMed
Assessing the efficacy of CRISPR/Cas9 genome editing in the wheat pathogen Parastagonspora nodorum. Khan H, McDonald MC, Williams SJ, Solomon PS. Fungal Biol Biotechnol. 2020 Mar 31;7:4. doi: 10.1186/s40694-020-00094-0. eCollection 2020. PubMed
The interdependence of mammary-specific super-enhancers and their native promoters facilitates gene activation during pregnancy. Zeng X, Lee HK, Wang C, Achikeh P, Liu C, Hennighausen L. Exp Mol Med. 2020 Apr;52(4):682-690. doi: 10.1038/s12276-020-0425-x. Epub 2020 Apr 22. PubMed
foxl3, a sexual switch in germ cells, initiates two independent molecular pathways for commitment to oogenesis in medaka. Kikuchi M, Nishimura T, Ishishita S, Matsuda Y, Tanaka M. Proc Natl Acad Sci U S A. 2020 Jun 2;117(22):12174-12181. doi: 10.1073/pnas.1918556117. Epub 2020 May 14. PubMed
Chaperone-Mediated Autophagy in the light of evolution: insight from fish. Lescat L, Veron V, Mourot B, Peron S, Chenais N, Dias K, Riera N, Beaumatin F, Pinel K, Priault M, Panserat S, Salin B, Guiguen Y, Bobe J, Herpin A, Seiliez I. Mol Biol Evol. 2020 May 21. pii: 5841670. doi: 10.1093/molbev/msaa127. PubMed
Pcdh18a regulates endocytosis of E-cadherin during axial mesoderm development in zebrafish. Bosze B, Ono Y, Mattes B, Sinner C, Gourain V, Thumberger T, Tlili S, Wittbrodt J, Saunders TE, Strahle U, Schug A, Scholpp S. Histochem Cell Biol. 2020 Jun 1. pii: 10.1007/s00418-020-01887-5. doi: 10.1007/s00418-020-01887-5. PubMed
Functional role of annexins in zebrafish caudal fin regeneration - A gene knockdown approach in regenerating tissue. Quoseena M, Vuppaladadium S, Hussain S, Banu S, Bharathi S, Idris MM. Biochimie. 2020 Aug;175:125-131. doi: 10.1016/j.biochi.2020.05.014. Epub 2020 Jun 16. PubMed
Deletion of the PDZ-binding kinase (Pbk) gene does not affect male fertility in mice. Miki Y, Devi L, Imai Y, Minami N, Koide T, Goel S. Reprod Fertil Dev. 2020 Jun;32(10):893-902. doi: 10.1071/RD19445. PubMed
Cerebral Dopamine Neurotrophic Factor Regulates Multiple Neuronal Subtypes and Behavior. Chen YC, Baronio D, Semenova S, Abdurakhmanova S, Panula P. J Neurosci. 2020 Aug 5;40(32):6146-6164. doi: 10.1523/JNEUROSCI.2636-19.2020. Epub 2020 Jul 6. PubMed
A Conserved Notochord Enhancer Controls Pancreas Development in Vertebrates. Amorim JP, Gali-Macedo A, Marcelino H, Bordeira-Carrico R, Naranjo S, Rivero-Gil S, Teixeira J, Galhardo M, Marques J, Bessa J. Cell Rep. 2020 Jul 7;32(1):107862. doi: 10.1016/j.celrep.2020.107862. PubMed
suz12 inactivation in p53- and nf1-deficient zebrafish accelerates the onset of malignant peripheral nerve sheath tumors and expands the spectrum of tumor types. Oppel F, Ki DH, Zimmerman MW, Ross KN, Tao T, Shi H, He S, Aster JC, Look AT. Dis Model Mech. 2020 Aug 27;13(8). pii: dmm.042341. doi: 10.1242/dmm.042341. PubMed
PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation. Johansson JA, Marie KL, Lu Y, Brombin A, Santoriello C, Zeng Z, Zich J, Gautier P, von Kriegsheim A, Brunsdon H, Wheeler AP, Dreger M, Houston DR, Dooley CM, Sims AH, Busch-Nentwich EM, Zon LI, Illingworth RS, Patton EE. Dev Cell. 2020 Aug 10;54(3):317-332.e9. doi: 10.1016/j.devcel.2020.06.013. Epub 2020 Jul 10. PubMed
Transcriptome analysis indicates dominant effects on ribosome and mitochondrial function of a premature termination codon mutation in the zebrafish gene psen2. Jiang H, Pederson SM, Newman M, Dong Y, Barthelson K, Lardelli M. PLoS One. 2020 Jul 13;15(7):e0232559. doi: 10.1371/journal.pone.0232559. eCollection 2020. PubMed
The zebrafish histamine H3 receptor modulates aggression, neural activity and forebrain functional connectivity. Reichmann F, Rimmer N, Tilley CA, Dalla Vecchia E, Pinion J, Al Oustah A, Carreno Gutierrez H, Young AMJ, McDearmid JR, Winter MJ, Norton WHJ. Acta Physiol (Oxf). 2020 Dec;230(4):e13543. doi: 10.1111/apha.13543. Epub 2020 Aug 12. PubMed
Transcriptional regulators of the Golli/myelin basic protein locus integrate additive and stealth activities. Bagheri H, Friedman H, Siminovitch KA, Peterson AC. PLoS Genet. 2020 Aug 13;16(8):e1008752. doi: 10.1371/journal.pgen.1008752. eCollection 2020 Aug. PubMed
ARID1A loss in neuroblastoma promotes the adrenergic-to-mesenchymal transition by regulating enhancer-mediated gene expression. Shi H, Tao T, Abraham BJ, Durbin AD, Zimmerman MW, Kadoch C, Look AT. Sci Adv. 2020 Jul 15;6(29):eaaz3440. doi: 10.1126/sciadv.aaz3440. eCollection 2020 Jul. PubMed
Production of a tributyltin-binding protein 2 knockout mutant strain of Japanese medaka, Oryzias latipes. Kato-Unoki Y, Takai Y, Nagano Y, Matsunaga S, Enoki S, Takamura T, Kim S, Kinoshita M, Kitano T, Shimasaki Y, Oshima Y. Mar Pollut Bull. 2020 Nov;160:111601. doi: 10.1016/j.marpolbul.2020.111601. Epub 2020 Aug 30. PubMed
Mouse models for V103I and I251L gain of function variants of the human MC4R display decreased adiposity but are not protected against a hypercaloric diet. Rojo D, McCarthy C, Raingo J, Rubinstein M. Mol Metab. 2020 Dec;42:101077. doi: 10.1016/j.molmet.2020.101077. Epub 2020 Sep 9. PubMed
The GEF Trio controls endothelial cell size and arterial remodeling downstream of Vegf signaling in both zebrafish and cell models. Klems A, van Rijssel J, Ramms AS, Wild R, Hammer J, Merkel M, Derenbach L, Preau L, Hinkel R, Suarez-Martinez I, Schulte-Merker S, Vidal R, Sauer S, Kivela R, Alitalo K, Kupatt C, van Buul JD, le Noble F. Nat Commun. 2020 Oct 21;11(1):5319. doi: 10.1038/s41467-020-19008-0. PubMed
Loss of Baiap2l2 destabilizes the transducing stereocilia of cochlear hair cells and leads to deafness. Carlton AJ, Halford J, Underhill A, Jeng JY, Avenarius MR, Gilbert ML, Ceriani F, Ebisine K, Brown SDM, Bowl MR, Barr-Gillespie PG, Marcotti W. J Physiol. 2021 Feb;599(4):1173-1198. doi: 10.1113/JP280670. Epub 2020 Nov 26. PubMed
CRISPR/Cas9 nickase-mediated efficient and seamless knock-in of lethal genes in the medaka fish Oryzias latipes. Murakami Y, Futamata R, Horibe T, Ueda K, Kinoshita M. Dev Growth Differ. 2020 Dec;62(9):554-567. doi: 10.1111/dgd.12700. PubMed
CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish. Amorim JP, Bordeira-Carrico R, Gali-Macedo A, Perrod C, Bessa J. STAR Protoc. 2020 Dec 10;1(3):100208. doi: 10.1016/j.xpro.2020.100208. eCollection 2020 Dec 18. PubMed
Venous Thrombosis and Thrombocyte Activity in Zebrafish Models of Quantitative and Qualitative Fibrinogen Disorders. Fish RJ, Freire C, Di Sanza C, Neerman-Arbez M. Int J Mol Sci. 2021 Jan 11;22(2). pii: ijms22020655. doi: 10.3390/ijms22020655. PubMed
Cystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in medaka. Arias Padilla LF, Castaneda-Cortes DC, Rosa IF, Moreno Acosta OD, Hattori RS, Nobrega RH, Fernandino JI. Elife. 2021 Mar 1;10. pii: 62757. doi: 10.7554/eLife.62757. PubMed
Knockout of zebrafish desmin genes does not cause skeletal muscle degeneration but alters calcium flux. Kayman Kurekci G, Kural Mangit E, Koyunlar C, Unsal S, Saglam B, Ergin B, Gizer M, Uyanik I, Boustanabadimaralan Duz N, Korkusuz P, Talim B, Purali N, Hughes SM, Dincer PR. Sci Rep. 2021 Apr 5;11(1):7505. doi: 10.1038/s41598-021-86974-w. PubMed
Sperm acrosome overgrowth and infertility in mice lacking chromosome 18 pachytene piRNA. Choi H, Wang Z, Dean J. PLoS Genet. 2021 Apr 8;17(4):e1009485. doi: 10.1371/journal.pgen.1009485. eCollection 2021 Apr. PubMed
Two novel mouse models mimicking minor deletions in 22q11.2 deletion syndrome revealed the contribution of each deleted region to psychiatric disorders. Saito R, Miyoshi C, Koebis M, Kushima I, Nakao K, Mori D, Ozaki N, Funato H, Yanagisawa M, Aiba A. Mol Brain. 2021 Apr 12;14(1):68. doi: 10.1186/s13041-021-00778-7. PubMed
Population scale nucleic acid delivery to Caenorhabditis elegans via electroporation. Khodakova AS, Vilchis DV, Blackburn D, Amanor F, Samuel BS. G3 (Bethesda). 2021 Apr 19. pii: 6238159. doi: 10.1093/g3journal/jkab123. PubMed
Characterization of PARP6 Function in Knockout Mice and Patients with Developmental Delay. Vermehren-Schmaedick A, Huang JY, Levinson M, Pomaville MB, Reed S, Bellus GA, Gilbert F, Keren B, Heron D, Haye D, Janello C, Makowski C, Danhauser K, Fedorov LM, Haack TB, Wright KM, Cohen MS. Cells. 2021 May 22;10(6). pii: cells10061289. doi: 10.3390/cells10061289. PubMed
foxm1 Modulates Cell Non-Autonomous Response in Zebrafish Skeletal Muscle Homeostasis. Ferreira FJ, Carvalho L, Logarinho E, Bessa J. Cells. 2021 May 18;10(5). pii: cells10051241. doi: 10.3390/cells10051241. PubMed
Puma, noxa, p53, and p63 differentially mediate stress pathway induced apoptosis. Wang J, Thomas HR, Li Z, Yeo NCF, Scott HE, Dang N, Hossain MI, Andrabi SA, Parant JM. Cell Death Dis. 2021 Jun 30;12(7):659. doi: 10.1038/s41419-021-03902-6. PubMed
A supernumerary "B-sex" chromosome drives male sex determination in the Pachon cavefish, Astyanax mexicanus. Imarazene B, Du K, Beille S, Jouanno E, Feron R, Pan Q, Torres-Paz J, Lopez-Roques C, Castinel A, Gil L, Kuchly C, Donnadieu C, Parrinello H, Journot L, Cabau C, Zahm M, Klopp C, Pavlica T, Al-Rikabi A, Liehr T, Simanovsky SA, Bohlen J, Sember A, Perez J, Veyrunes F, Mueller TD, Postlethwait JH, Schartl M, Herpin A, Retaux S, Guiguen Y. Curr Biol. 2021 Nov 8;31(21):4800-4809.e9. doi: 10.1016/j.cub.2021.08.030. Epub 2021 Sep 7. PubMed
Carrier-Free Cellular Transport of CRISPR/Cas9 Ribonucleoprotein for Genome Editing by Cold Atmospheric Plasma. Cui H, Jiang M, Zhou W, Gao M, He R, Huang Y, Chu PK, Yu XF. Biology (Basel). 2021 Oct 13;10(10). pii: biology10101038. doi: 10.3390/biology10101038. PubMed
Loss of Neuropilin2a/b or Sema3fa alters olfactory sensory axon dynamics and protoglomerular targeting. Cheng RP, Dang P, Taku AA, Moon YJ, Pham V, Sun X, Zhao E, Raper JA. Neural Dev. 2022 Jan 3;17(1):1. doi: 10.1186/s13064-021-00157-x. PubMed
Disruption of Epidermal Growth Factor Receptor but Not EGF Blocks Follicle Activation in Zebrafish Ovary. Song Y, Chen W, Zhu B, Ge W. Front Cell Dev Biol. 2022 Jan 17;9:750888. doi: 10.3389/fcell.2021.750888. eCollection 2021. PubMed
Hemoglobin in the blood acts as a chemosensory signal via the mouse vomeronasal system. Osakada T, Abe T, Itakura T, Mori H, Ishii KK, Eguchi R, Murata K, Saito K, Haga-Yamanaka S, Kimoto H, Yoshihara Y, Miyamichi K, Touhara K. Nat Commun. 2022 Feb 3;13(1):556. doi: 10.1038/s41467-022-28118-w. PubMed
ANKRD24 organizes TRIOBP to reinforce stereocilia insertion points. Krey JF, Liu C, Belyantseva IA, Bateschell M, Dumont RA, Goldsmith J, Chatterjee P, Morrill RS, Fedorov LM, Foster S, Kim J, Nuttall AL, Jones SM, Choi D, Friedman TB, Ricci AJ, Zhao B, Barr-Gillespie PG. J Cell Biol. 2022 Apr 4;221(4). pii: 213014. doi: 10.1083/jcb.202109134. Epub 2022 Feb 17. PubMed
A Zebrafish Model for a Rare Genetic Disease Reveals a Conserved Role for FBXL3 in the Circadian Clock System. Confino S, Dor T, Tovin A, Wexler Y, Ben-Moshe Livne Z, Kolker M, Pisanty O, Park SK, Geyer N, Reiter J, Edvardson S, Mor-Shaked H, Elpeleg O, Vallone D, Appelbaum L, Foulkes NS, Gothilf Y. Int J Mol Sci. 2022 Feb 21;23(4). pii: ijms23042373. doi: 10.3390/ijms23042373. PubMed
Development of a screening system for agents that modulate taste receptor expression with the CRISPR-Cas9 system in medaka. Beppu K, Tsutsumi R, Ansai S, Ochiai N, Terakawa M, Mori M, Kuroda M, Horikawa K, Tomoi T, Sakamoto J, Kamei Y, Naruse K, Sakaue H. Biochem Biophys Res Commun. 2022 Apr 23;601:65-72. doi: 10.1016/j.bbrc.2022.02.082. Epub 2022 Feb 23. PubMed
Genetic and Neurological Deficiencies in the Visual System of mct8 Mutant Zebrafish. Rozenblat R, Tovin A, Zada D, Lebenthal-Loinger I, Lerer-Goldshtein T, Appelbaum L. Int J Mol Sci. 2022 Feb 23;23(5). pii: ijms23052464. doi: 10.3390/ijms23052464. PubMed
Oxytocin receptors influence the development and maintenance of social behavior in zebrafish (Danio rerio). Gemmer A, Mirkes K, Anneser L, Eilers T, Kibat C, Mathuru A, Ryu S, Schuman E. Sci Rep. 2022 Mar 12;12(1):4322. doi: 10.1038/s41598-022-07990-y. PubMed
Boosting targeted genome editing using the hei-tag. Thumberger T, Tavhelidse-Suck T, Gutierrez-Triana JA, Cornean A, Medert R, Welz B, Freichel M, Wittbrodt J. Elife. 2022 Mar 25;11. pii: 70558. doi: 10.7554/eLife.70558. PubMed

Associated Plasmids
Precise in vivo functional analysis of DNA variants with base editing using ACEofBASEs target prediction. Cornean A, Gierten J, Welz B, Mateo JL, Thumberger T, Wittbrodt J. Elife. 2022 Apr 4;11:e72124. doi: 10.7554/eLife.72124. PubMed
Multidimensional chromatin profiling of zebrafish pancreas to uncover and investigate disease-relevant enhancers. Bordeira-Carrico R, Teixeira J, Duque M, Galhardo M, Ribeiro D, Acemel RD, Firbas PN, Tena JJ, Eufrasio A, Marques J, Ferreira FJ, Freitas T, Carneiro F, Gomez-Skarmeta JL, Bessa J. Nat Commun. 2022 Apr 11;13(1):1945. doi: 10.1038/s41467-022-29551-7. PubMed
Inhibition of Crmp1 Phosphorylation at Ser522 Ameliorates Motor Function and Neuronal Pathology in Amyotrophic Lateral Sclerosis Model Mice. Asano T, Nakamura H, Kawamoto Y, Tada M, Kimura Y, Takano H, Yao R, Saito H, Ikeda T, Komiya H, Kubota S, Hashiguchi S, Takahashi K, Kunii M, Tanaka K, Goshima Y, Nakamura F, Takeuchi H, Doi H, Tanaka F. eNeuro. 2022 May 23;9(3). pii: ENEURO.0133-22.2022. doi: 10.1523/ENEURO.0133-22.2022. Print 2022 May-Jun. PubMed
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