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Zebrafish-gRNA-0004
(Plasmid #42244)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42244 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    DR274
  • Backbone manufacturer
    Joung lab (Addgene plasmid# 42250)
  • Backbone size w/o insert (bp) 2147
  • Vector type
    CRISPR ; zebrafish expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRNA-fh site #2
  • Alt name
    DR302
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    20
  • Entrez Gene
    fh (a.k.a. Fh1, im:7152785, ns:zf-e152, zf-e152, zgc:66253, zgc:77498)
  • Promoter T7

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Target site: GGAGCGAGCGGAGCGGTACA

Guide RNA sequence: TAATACGACTCACTATAGGAGCGAGCGGAGCGGTACAGTTTTAGAGCTAGAAATAGCAAGT TAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTAAA

For more information on Joung Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/jounglab/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Zebrafish-gRNA-0004 was a gift from Keith Joung (Addgene plasmid # 42244 ; http://n2t.net/addgene:42244 ; RRID:Addgene_42244)

  • For your References section:

    Efficient genome editing in zebrafish using a CRISPR-Cas system. Hwang WY, Fu Y, Reyon D, Maeder ML, Tsai SQ, Sander JD, Peterson RT, Yeh JR, Joung JK. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2501. 10.1038/nbt.2501 PubMed 23360964
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