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pENTR-3xFLAG-YAP1-S127A
(Plasmid #42239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEntr3c
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2300
  • Total vector size (bp) 3800
  • Modifications to backbone
    inserted 3xFLAG-YAP1-S127A. Has stop codon.
  • Vector type
    gateway

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YAP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1500
  • Mutation
    Serine 127 changed to alanine
  • Entrez Gene
    YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
  • Promoter none
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer TCGGGCCCCAAATAATGA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-3xFLAG-YAP1-S127A was a gift from William Pu (Addgene plasmid # 42239 ; http://n2t.net/addgene:42239 ; RRID:Addgene_42239)

  • For your References section:

    YAP1, the nuclear target of Hippo signaling, stimulates heart growth through cardiomyocyte proliferation but not hypertrophy. von Gise A, Lin Z, Schlegelmilch K, Honor LB, Pan GM, Buck JN, Ma Q, Ishiwata T, Zhou B, Camargo FD, Pu WT. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2394-9. doi: 10.1073/pnas.1116136109. Epub 2012 Jan 30. 10.1073/pnas.1116136109 PubMed 22308401
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