pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro Citations (34)
Originally described in: Multiplex Genome Engineering Using CRISPR/Cas Systems.Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F Science. 2013 Jan 3. PubMed Journal
Articles Citing pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
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| RNA-guided genome editing for target gene mutations in wheat. Upadhyay SK, Kumar J, Alok A, Tuli R. G3 (Bethesda). 2013 Dec 9;3(12):2233-8. doi: 10.1534/g3.113.008847. PubMed |
| Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system. Zhao Y, Dai Z, Liang Y, Yin M, Ma K, He M, Ouyang H, Teng CB. Sci Rep. 2014 Feb 3;4:3943. doi: 10.1038/srep03943. PubMed |
An iCRISPR Platform for Rapid, Multiplexable, and Inducible Genome Editing in Human Pluripotent Stem Cells.
Gonzalez F, Zhu Z, Shi ZD, Lelli K, Verma N, Li QV, Huangfu D.
Cell Stem Cell. 2014 Jun 11. pii: S1934-5909(14)00205-7. doi: 10.1016/j.stem.2014.05.018.
PubMed
Associated Plasmids |
Mouse Genome Editing Using the CRISPR/Cas System.
Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, Gurumurthy CB.
Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 10.1002/0471142905.hg1507s83.
PubMed
Associated Plasmids |
| Inhibition of HSV-1 Replication by Gene Editing Strategy. Roehm PC, Shekarabi M, Wollebo HS, Bellizzi A, He L, Salkind J, Khalili K. Sci Rep. 2016 Apr 11;6:23146. doi: 10.1038/srep23146. PubMed |
| Loss of ubiquitin E2 Ube2w rescues hypersensitivity of Rnf4 mutant cells to DNA damage. Maure JF, Moser SC, Jaffray EG, F Alpi A, Hay RT. Sci Rep. 2016 May 17;6:26178. doi: 10.1038/srep26178. PubMed |
| The MYC 3' Wnt-Responsive Element Drives Oncogenic MYC Expression in Human Colorectal Cancer Cells. Rennoll SA, Eshelman MA, Raup-Konsavage WM, Kawasawa YI, Yochum GS. Cancers (Basel). 2016 May 23;8(5). pii: E52. doi: 10.3390/cancers8050052. PubMed |
| An RNA-aptamer-based two-color CRISPR labeling system. Wang S, Su JH, Zhang F, Zhuang X. Sci Rep. 2016 May 27;6:26857. doi: 10.1038/srep26857. PubMed |
| Cytoplasmic Drosha activity generated by alternative splicing. Dai L, Chen K, Youngren B, Kulina J, Yang A, Guo Z, Li J, Yu P, Gu S. Nucleic Acids Res. 2016 Dec 1;44(21):10454-10466. Epub 2016 Jul 28. PubMed |
| Negative Feedback Regulation of HIV-1 by Gene Editing Strategy. Kaminski R, Chen Y, Salkind J, Bella R, Young WB, Ferrante P, Karn J, Malcolm T, Hu W, Khalili K. Sci Rep. 2016 Aug 16;6:31527. doi: 10.1038/srep31527. PubMed |
| Polycistronic tRNA and CRISPR guide-RNA enables highly efficient multiplexed genome engineering in human cells. Dong F, Xie K, Chen Y, Yang Y, Mao Y. Biochem Biophys Res Commun. 2017 Jan 22;482(4):889-895. doi: 10.1016/j.bbrc.2016.11.129. Epub 2016 Nov 25. PubMed |
| Astrocyte pathology in a human neural stem cell model of frontotemporal dementia caused by mutant TAU protein. Hallmann AL, Arauzo-Bravo MJ, Mavrommatis L, Ehrlich M, Ropke A, Brockhaus J, Missler M, Sterneckert J, Scholer HR, Kuhlmann T, Zaehres H, Hargus G. Sci Rep. 2017 Mar 3;7:42991. doi: 10.1038/srep42991. PubMed |
| Dissection of mechanical force in living cells by super-resolved traction force microscopy. Colin-York H, Eggeling C, Fritzsche M. Nat Protoc. 2017 Apr;12(4):783-796. doi: 10.1038/nprot.2017.009. Epub 2017 Mar 16. PubMed |
| Phosphorylated SIRT1 associates with replication origins to prevent excess replication initiation and preserve genomic stability. Utani K, Fu H, Jang SM, Marks AB, Smith OK, Zhang Y, Redon CE, Shimizu N, Aladjem MI. Nucleic Acids Res. 2017 May 26. doi: 10.1093/nar/gkx468. PubMed |
| ANGPTL4 T266M variant is associated with reduced cancer invasiveness. Tan ZW, Teo Z, Tan C, Choo CC, Loo WS, Song Y, Tam ZY, Ng SP, Koh HZ, Ng YS, Shochat SG, Yau YH, Zhu P, Tan NS. Biochim Biophys Acta. 2017 Jun 19;1864(10):1525-1536. doi: 10.1016/j.bbamcr.2017.06.010. PubMed |
| Optimized guide RNA structure for genome editing via Cas9. Xu J, Lian W, Jia Y, Li L, Huang Z. Oncotarget. 2017 Oct 7;8(55):94166-94171. doi: 10.18632/oncotarget.21607. eCollection 2017 Nov 7. PubMed |
| CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy. Yao X, Wang X, Liu J, Shi L, Huang P, Yang H. J Vis Exp. 2018 Mar 12;(133). doi: 10.3791/56844. PubMed |
| Tild-CRISPR Allows for Efficient and Precise Gene Knockin in Mouse and Human Cells. Yao X, Zhang M, Wang X, Ying W, Hu X, Dai P, Meng F, Shi L, Sun Y, Yao N, Zhong W, Li Y, Wu K, Li W, Chen ZJ, Yang H. Dev Cell. 2018 May 21;45(4):526-536.e5. doi: 10.1016/j.devcel.2018.04.021. PubMed |
| Cell surface flip-flop of phosphatidylserine is critical for PIEZO1-mediated myotube formation. Tsuchiya M, Hara Y, Okuda M, Itoh K, Nishioka R, Shiomi A, Nagao K, Mori M, Mori Y, Ikenouchi J, Suzuki R, Tanaka M, Ohwada T, Aoki J, Kanagawa M, Toda T, Nagata Y, Matsuda R, Takayama Y, Tominaga M, Umeda M. Nat Commun. 2018 May 24;9(1):2049. doi: 10.1038/s41467-018-04436-w. PubMed |
| Mitoregulin: A lncRNA-Encoded Microprotein that Supports Mitochondrial Supercomplexes and Respiratory Efficiency. Stein CS, Jadiya P, Zhang X, McLendon JM, Abouassaly GM, Witmer NH, Anderson EJ, Elrod JW, Boudreau RL. Cell Rep. 2018 Jun 26;23(13):3710-3720.e8. doi: 10.1016/j.celrep.2018.06.002. PubMed |
| Optimized Plasmid Construction Strategy for Cas9. Xu J, Li W, Hossen MM, Jia Y, Li L, Huang Z. Cell Physiol Biochem. 2018;48(1):131-137. doi: 10.1159/000491669. Epub 2018 Jul 12. PubMed |
| Zinc-Finger Nucleases Induced by HIV-1 Tat Excise HIV-1 from the Host Genome in Infected and Latently Infected Cells. Ji H, Lu P, Liu B, Qu X, Wang Y, Jiang Z, Yang X, Zhong Y, Yang H, Pan H, Zhao L, Xu J, Lu H, Zhu H. Mol Ther Nucleic Acids. 2018 Sep 7;12:67-74. doi: 10.1016/j.omtn.2018.04.014. Epub 2018 May 3. PubMed |
Regulation of Neuroregeneration by Long Noncoding RNAs.
Perry RB, Hezroni H, Goldrich MJ, Ulitsky I.
Mol Cell. 2018 Nov 1;72(3):553-567.e5. doi: 10.1016/j.molcel.2018.09.021. Epub 2018 Oct 25.
PubMed
Associated Plasmids |
| Genetic Models Reveal cis and trans Immune-Regulatory Activities for lincRNA-Cox2. Elling R, Robinson EK, Shapleigh B, Liapis SC, Covarrubias S, Katzman S, Groff AF, Jiang Z, Agarwal S, Motwani M, Chan J, Sharma S, Hennessy EJ, FitzGerald GA, McManus MT, Rinn JL, Fitzgerald KA, Carpenter S. Cell Rep. 2018 Nov 6;25(6):1511-1524.e6. doi: 10.1016/j.celrep.2018.10.027. PubMed |
| P53 ICE CRIM mouse: a tool to generate mutant allelic series in somatic cells and germ lines for cancer studies. Fan HH, Yu IS, Lin YH, Wang SY, Liaw YH, Chen PL, Yang TL, Lin SW, Chen YT. FASEB J. 2019 Apr;33(4):5571-5584. doi: 10.1096/fj.201802027R. Epub 2019 Jan 14. PubMed |
| Genomic Structure of Hstx2 Modifier of Prdm9-Dependent Hybrid Male Sterility in Mice. Lustyk D, Kinsky S, Ullrich KK, Yancoskie M, Kasikova L, Gergelits V, Sedlacek R, Chan YF, Odenthal-Hesse L, Forejt J, Jansa P. Genetics. 2019 Nov;213(3):1047-1063. doi: 10.1534/genetics.119.302554. Epub 2019 Sep 27. PubMed |
| In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system. Chen Y, Jiang H, Wang T, He D, Tian R, Cui Z, Tian X, Gao Q, Ma X, Yang J, Wu J, Tan S, Xu H, Tang X, Wang Y, Yu Z, Han H, Das BC, Severinov K, Hitzeroth II, Debata PR, Xu W, Fan W, Jin Z, Cao C, Yu M, Xie W, Huang Z, Hu Z, You Z. Antiviral Res. 2020 Jun;178:104794. doi: 10.1016/j.antiviral.2020.104794. Epub 2020 Apr 14. PubMed |
| Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes. Wang G, Li C, He S, Liu Z. Development. 2021 Mar 9;148(5). pii: dev.196899. doi: 10.1242/dev.196899. PubMed |
| The comparison of ZFNs, TALENs, and SpCas9 by GUIDE-seq in HPV-targeted gene therapy. Cui Z, Liu H, Zhang H, Huang Z, Tian R, Li L, Fan W, Chen Y, Chen L, Zhang S, Das BC, Severinov K, Hitzeroth II, Debata PR, Jin Z, Liu J, Huang Z, Xie W, Xie H, Lang B, Ma J, Weng H, Tian X, Hu Z. Mol Ther Nucleic Acids. 2021 Aug 19;26:1466-1478. doi: 10.1016/j.omtn.2021.08.008. eCollection 2021 Dec 3. PubMed |
| FrCas9 is a CRISPR/Cas9 system with high editing efficiency and fidelity. Cui Z, Tian R, Huang Z, Jin Z, Li L, Liu J, Huang Z, Xie H, Liu D, Mo H, Zhou R, Lang B, Meng B, Weng H, Hu Z. Nat Commun. 2022 Mar 17;13(1):1425. doi: 10.1038/s41467-022-29089-8. PubMed |
| Cytoplasmic PARP1 links the genome instability to the inhibition of antiviral immunity through PARylating cGAS. Wang F, Zhao M, Chang B, Zhou Y, Wu X, Ma M, Liu S, Cao Y, Zheng M, Dang Y, Xu J, Chen L, Liu T, Tang F, Ren Y, Xu Z, Mao Z, Huang K, Luo M, Li J, Liu H, Ge B. Mol Cell. 2022 Jun 2;82(11):2032-2049.e7. doi: 10.1016/j.molcel.2022.03.034. Epub 2022 Apr 22. PubMed |
| TAK1 is an essential kinase for STING trafficking. Ma M, Dang Y, Chang B, Wang F, Xu J, Chen L, Su H, Li J, Ge B, Chen C, Liu H. Mol Cell. 2023 Nov 2;83(21):3885-3903.e5. doi: 10.1016/j.molcel.2023.09.009. Epub 2023 Oct 12. PubMed |
| CRISPR/Cas9 Genome Editing in LGMD2A/R1 Patient-Derived Induced Pluripotent Stem and Skeletal Muscle Progenitor Cells. Mavrommatis L, Zaben A, Kindler U, Kienitz MC, Dietz J, Jeong HW, Bohme P, Brand-Saberi B, Vorgerd M, Zaehres H. Stem Cells Int. 2023 Nov 9;2023:9246825. doi: 10.1155/2023/9246825. eCollection 2023. PubMed |
| Mycobacterium tuberculosis inhibits METTL14-mediated m(6)A methylation of Nox2 mRNA and suppresses anti-TB immunity. Ma M, Duan Y, Peng C, Wu Y, Zhang X, Chang B, Wang F, Yang H, Zheng R, Cheng H, Cheng Y, He Y, Huang J, Lei J, Ma H, Li L, Wang J, Huang X, Tang F, Liu J, Li J, Ying R, Wang P, Sha W, Gao Y, Wang L, Ge B. Cell Discov. 2024 Mar 29;10(1):36. doi: 10.1038/s41421-024-00653-4. PubMed |
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