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Addgene

pMQ430
(Plasmid #42216)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42216 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD18
  • Total vector size (bp) 5450
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dam
  • Species
    E. coli K-12
  • Insert Size (bp)
    842
  • GenBank ID
    NC_000913
  • Entrez Gene
    dam (a.k.a. b3387, ECK3374)
  • Promoter pBAD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMQ430 was a gift from Martin Marinus (Addgene plasmid # 42216 ; http://n2t.net/addgene:42216 ; RRID:Addgene_42216)

  • For your References section:

    Regulated expression of the Escherichia coli dam gene. Calmann MA, Marinus MG. J Bacteriol. 2003 Aug;185(16):5012-4. 10.1128/JB.185.16.5012-5014.2003 PubMed 12897023
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