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Addgene

pcDNA3 c-SRC (250-536) HA
(Plasmid #42209)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42209 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 7000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    c-SRC (250-536) HA
  • Alt name
    SRC
  • Alt name
    sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
  • Alt name
    p60-Src
  • Species
    H. sapiens (human)
  • Mutation
    contains amino acids 250-536
  • GenBank ID
    NM_005417.3 AAA60584.1
  • Entrez Gene
    SRC (a.k.a. ASV, SRC1, THC6, c-SRC, p60-Src)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

All c-SRC mutants were generated by polymerase chain reaction using c-SRC (WT) (Addgene Plasmid #42202) as a template, and polymerase chain reaction products containing a minimal Kozak sequence were cloned into the HindIII and XhoI sites of pcDNA3. This construct contains amino acids 250-536 of human c-SRC followed by a carboxyl-terminal HA tag. All sequences were confirmed using an automated ABI DNA sequencer (Howard Hughes Nucleic Acid Facility, Duke University).

Alternate plasmid name: pcDNA3 c-SRC-SH1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 c-SRC (250-536) HA was a gift from Robert Lefkowitz (Addgene plasmid # 42209 ; http://n2t.net/addgene:42209 ; RRID:Addgene_42209)

  • For your References section:

    beta-arrestin1 interacts with the catalytic domain of the tyrosine kinase c-SRC. Role of beta-arrestin1-dependent targeting of c-SRC in receptor endocytosis. Miller WE, Maudsley S, Ahn S, Khan KD, Luttrell LM, Lefkowitz RJ. J Biol Chem. 2000 Apr 14;275(15):11312-9. 10.1074/jbc.275.15.11312 PubMed 10753943
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