pcDNA3 c-SRC (151-536)
(Plasmid #42207)

• Depositing Lab: Robert Lefkowitz
• Publication: Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Total vector size (bp): 7000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: c-SRC (151-536)
• Alt name: SRC
• Alt name: sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
• Alt name: p60-Src
• Species: H. sapiens (human)
• Mutation: contains amino acids 151-536
• GenBank ID: NM_005417.3 AAA60584.1
• Entrez Gene: SRC (a.k.a. ASV, SRC1, THC6, c-SRC, p60-Src)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

All c-SRC mutants were generated by polymerase chain reaction using c-SRC (WT) (Addgene Plasmid #42202) as a template, and polymerase chain reaction products containing a minimal Kozak sequence were cloned into the HindIII and XhoI sites of pcDNA3. This construct contains amino acids 151-536 of human c-SRC and is untagged. All sequences were confirmed using an automated ABI DNA sequencer (Howard Hughes Nucleic Acid Facility, Duke University).

This plasmid also contains an L176F point mutation which should not affect function.