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Addgene

Venus-Gprotein Gamma 9
(Plasmid #42201)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42201 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6110
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G protein Gamma 9
  • Alt name
    Gng9
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    219
  • GenBank ID
    NM_174328.2
  • Promoter CMV
  • Tag / Fusion Protein
    • Venus(1-155) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cutting with BamH1 and XbaI releases the gamma 9 fragment

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Venus-Gprotein Gamma 9 was a gift from Narasimhan Gautam (Addgene plasmid # 42201 ; http://n2t.net/addgene:42201 ; RRID:Addgene_42201)

  • For your References section:

    All G protein betagamma complexes are capable of translocation on receptor activation. Ajith Karunarathne WK, O'Neill PR, Martinez-Espinosa PL, Kalyanaraman V, Gautam N. Biochem Biophys Res Commun. 2012 May 11;421(3):605-11. doi: 10.1016/j.bbrc.2012.04.054. Epub 2012 Apr 19. 10.1016/j.bbrc.2012.04.054 PubMed 22538369
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