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Addgene

Venus-G protein Gamma 5
(Plasmid #42200)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42200 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 5631
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gamma 5
  • Alt name
    Gng5
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    204
  • Mutation
    codon-optimized
  • GenBank ID
    NM_024377.1
  • Entrez Gene
    Gng5
  • Promoter CMV
  • Tag / Fusion Protein
    • Venus(1-155) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Venus-G protein Gamma 5 was a gift from Narasimhan Gautam (Addgene plasmid # 42200 ; http://n2t.net/addgene:42200 ; RRID:Addgene_42200)

  • For your References section:

    All G protein betagamma complexes are capable of translocation on receptor activation. Ajith Karunarathne WK, O'Neill PR, Martinez-Espinosa PL, Kalyanaraman V, Gautam N. Biochem Biophys Res Commun. 2012 May 11;421(3):605-11. doi: 10.1016/j.bbrc.2012.04.054. Epub 2012 Apr 19. 10.1016/j.bbrc.2012.04.054 PubMed 22538369
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