pRc/CMV2 Dvl-2-Myc
(Plasmid #42194)

• Depositing Labs: Robert Lefkowitz, Shin-ichi Yanagawa
• Publication: Lee et al J Biol Chem. 1999 Jul 23;274(30):21464-70.

Backbone

• Vector backbone: pRc/CMV2
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dvl 2
• Alt name: dishevelled 2, dsh homolog
• Alt name: Dvl2
• Alt name: Dvl-2
• Species: M. musculus (mouse)
• GenBank ID: NM_007888.3 NP_031914.3
• Entrez Gene: Dvl2
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: HindIII (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dvl-2 sequence was amplified by PCR using a pBluescript IISK plasmid containing the entire coding sequence of mouse Dvl-2 (from Daniel J. Sussman, University of Maryland School of Medicine, Baltimore, MD) as the template.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The resulting PCR described above encompasses amino acids 1-679 of the current NCBI sequence.