pMyNT
(Plasmid #42191)

• Purpose: (Empty Backbone)
• Depositing Labs: Annabel Parret, Matthias Wilmanns
• Publication: Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.

Backbone

• Vector backbone: pSD26
• Backbone manufacturer: Daugelat et al 2003
• Backbone size (bp): 6900
• Modifications to backbone: Vector backbone corresponds to the pSD26 plasmid [Daugelat et al 2003] with two modifications: (i) deleting the six initial codons of acetamidase, (ii) inserting a multiple cloning site (MCS) consisting of restriction endonuclease sites, NcoI, KpnI, BamHI, EcoRI, EcoRV and HindIII. Middle:
• Vector type: Bacterial Expression
• Promoter: ColE1
• Tag / Fusion Protein:
  • His6 (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • pMyNT gb file
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Vector constructed by Dr. Arie Geerlof at EMBL-Hamburg (now at Helmholtz Zentrum München)