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Addgene

EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1
(Plasmid #42188)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42188 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 5653
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gamma 3(K68A,K69A)
  • Alt name
    Gng3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    225
  • Mutation
    K68A,K69A
  • GenBank ID
    NM_010316.3
  • Entrez Gene
    Gng3
  • Promoter cmv
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutations created by using the Quik Change mutagenesis kit

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1 was a gift from Narasimhan Gautam (Addgene plasmid # 42188 ; http://n2t.net/addgene:42188 ; RRID:Addgene_42188)

  • For your References section:

    G-protein signaling leverages subunit-dependent membrane affinity to differentially control betagamma translocation to intracellular membranes. O'Neill PR, Karunarathne WK, Kalyanaraman V, Silvius JR, Gautam N. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):E3568-77. doi: 10.1073/pnas.1205345109. Epub 2012 Dec 3. 10.1073/pnas.1205345109 PubMed 23213235
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