EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1
(Plasmid
#42185)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 5653
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameG protein Gamma 3(F70A, F71A)
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Alt nameGng3
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SpeciesM. musculus (mouse)
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Insert Size (bp)225
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MutationF70A, F71A
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GenBank IDNM_010316.3
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Entrez GeneGng3
- Promoter cmv
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T7 primer
- 3′ sequencing primer BGH primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Mutations created by using the Quik Change Mutagenesis kit.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1 was a gift from Narasimhan Gautam (Addgene plasmid # 42185 ; http://n2t.net/addgene:42185 ; RRID:Addgene_42185) -
For your References section:
G-protein signaling leverages subunit-dependent membrane affinity to differentially control betagamma translocation to intracellular membranes. O'Neill PR, Karunarathne WK, Kalyanaraman V, Silvius JR, Gautam N. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):E3568-77. doi: 10.1073/pnas.1205345109. Epub 2012 Dec 3. 10.1073/pnas.1205345109 PubMed 23213235
Map uploaded by the depositor.
