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Addgene

pNH090
(Plasmid #42166)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42166 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCC1Fos
  • Backbone manufacturer
    Eipcentre
  • Backbone size w/o insert (bp) 8353
  • Total vector size (bp) 9291

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Growth instructions
    pCC1Fos-based 'CopyControl' construct enabling transient induction of copy-number
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mTFP1::[(G4S)3]::C-TAP Tag
  • Species
    C. elegans (nematode), Synthetic
  • Insert Size (bp)
    938

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNH090 was a gift from Colin Dolphin (Addgene plasmid # 42166 ; http://n2t.net/addgene:42166 ; RRID:Addgene_42166)

  • For your References section:

    A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT. BMC Biotechnol. 2013 Jan 3;13(1):1. 10.1186/1472-6750-13-1 PubMed 23281894
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