pNH082
(Plasmid
#42165)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42165 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGOv5
- Backbone size w/o insert (bp) 2131
- Total vector size (bp) 2997
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTFP1
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SpeciesC. elegans (nematode), Synthetic
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Insert Size (bp)866
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNH082 was a gift from Colin Dolphin (Addgene plasmid # 42165 ; http://n2t.net/addgene:42165 ; RRID:Addgene_42165) -
For your References section:
A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT. BMC Biotechnol. 2013 Jan 3;13(1):1. 10.1186/1472-6750-13-1 PubMed 23281894
Map uploaded by the depositor.
Map uploaded by the depositor.
