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Addgene

pNH002
(Plasmid #42145)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42145 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGOv5
  • Backbone manufacturer
    GeneOracle
  • Backbone size w/o insert (bp) 2172
  • Total vector size (bp) 3075
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-TAP TAG::[(G4S)3]::mTFP1
  • Species
    C. elegans (nematode), Synthetic
  • Insert Size (bp)
    903
  • Tag / Fusion Protein
    • TAP TAG (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Insert synthesized by GeneOracle in pGOv5

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Nb. CDS terminates with two in-frame stop codons [TAA-TAG]

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNH002 was a gift from Colin Dolphin (Addgene plasmid # 42145 ; http://n2t.net/addgene:42145 ; RRID:Addgene_42145)

  • For your References section:

    A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT. BMC Biotechnol. 2013 Jan 3;13(1):1. 10.1186/1472-6750-13-1 PubMed 23281894
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