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Addgene

pMYC-GFP
(Plasmid #42142)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42142 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1/CT-GFP-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6157
  • Total vector size (bp) 7482
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    c-Myc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1325
  • GenBank ID
    BC000917
  • Entrez Gene
    MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer TCCCGCGACG ATGCCCCTCA ACGTTAGCTTCA
  • 3′ sequencing primer CACAAGAGTT CCGTAGCTGT TCAAGTTTGTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMYC-GFP was a gift from Olufunmilayo Olopade (Addgene plasmid # 42142 ; http://n2t.net/addgene:42142 ; RRID:Addgene_42142)

  • For your References section:

    c-Myc activates BRCA1 gene expression through distal promoter elements in breast cancer cells. Chen Y, Xu J, Borowicz S, Collins C, Huo D, Olopade OI. BMC Cancer. 2011 Jun 13;11:246. doi: 10.1186/1471-2407-11-246. 10.1186/1471-2407-11-246 PubMed 21668996
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