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pET21b-RL016A
(Plasmid #42135)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42135 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5367
  • Total vector size (bp) 6843
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mYPet-mCherry
  • Alt name
    A206K YPet-mCherry
  • Species
    Synthetic
  • Insert Size (bp)
    1476
  • Promoter T7 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer ctctctGGATCCgaataattttgtttaactttaagaag
  • 3′ sequencing primer gtgtgtCTCGAGttatttatacagctcatccata
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The first gene is mYPet not Venus as shown in the plasmid map generated by the website. Please note that the G11S mutation in YPet does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21b-RL016A was a gift from Sheref Mansy (Addgene plasmid # 42135 ; http://n2t.net/addgene:42135 ; RRID:Addgene_42135)

  • For your References section:

    Fluorescent Proteins and in Vitro Genetic Organization for Cell-Free Synthetic Biology. Lentini R, Forlin M, Martini L, Del Bianco C, Spencer AC, Torino D, Mansy SS. ACS Synth Biol. 2013 Mar 15. 10.1021/sb400003y PubMed 23654270
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