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pQC_HA-p115 TEV-Myc #77#407
(Plasmid #42127)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42127 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIN
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p115
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    2200
  • Mutation
    inserted ENLYFQ b/w 886 and 887
  • Promoter CMV
  • Tags / Fusion Proteins
    • Myc (C terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQC_HA-p115 TEV-Myc #77#407 was a gift from Ayano Satoh (Addgene plasmid # 42127 ; http://n2t.net/addgene:42127 ; RRID:Addgene_42127)

  • For your References section:

    Detection of in situ cleaved p115 with the cut specific antibodies in rapid protein inactivation system by tobacco etch viral protease cleavage. Koreishi M, Honjo Y, Satoh A. Antibody Technology Journal. 2011;1:5-11 10.2147/ANTI.S22825
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