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pQC-SEAP #M004#725
(Plasmid #42125)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42125 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIH
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7200
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alkaline phosphatase, placental (SEAP)
  • Alt name
    ALPP
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2883
  • Entrez Gene
    ALPP (a.k.a. ALP, ALPI, IAP, PALP, PLAP, PLAP-1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQC-SEAP #M004#725 was a gift from Ayano Satoh (Addgene plasmid # 42125 ; http://n2t.net/addgene:42125 ; RRID:Addgene_42125)

  • For your References section:

    CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking. Koreishi M, Yu S, Oda M, Honjo Y, Satoh A. PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 2013 Jan 18. 10.1371/journal.pone.0054382 PubMed 23349870
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