GFP-Sec31a 4SA #33#638
(Plasmid
#42124)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 42124 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQCXIP
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 7200
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSec31a
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SpeciesH. sapiens (human)
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Insert Size (bp)3700
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MutationS527A S799A S1163A T1165A
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Entrez GeneSEC31A (a.k.a. ABP125, ABP130, HPBKS, HSPC275, HSPC334, NEDSOSB, SEC31L1)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer EGFPC
- 3′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-Sec31a 4SA #33#638 was a gift from Ayano Satoh (Addgene plasmid # 42124 ; http://n2t.net/addgene:42124 ; RRID:Addgene_42124) -
For your References section:
CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking. Koreishi M, Yu S, Oda M, Honjo Y, Satoh A. PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 2013 Jan 18. 10.1371/journal.pone.0054382 PubMed 23349870