Skip to main content
Addgene

FLAG-Sec31a W985A #790
(Plasmid #42122)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFLAG-CMV
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sec31a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3700
  • Mutation
    G742R and W985A NOTE: the mutation is actually W995A See Comments.
  • Entrez Gene
    SEC31A (a.k.a. ABP125, ABP130, HPBKS, HSPC275, HSPC334, NEDSOSB, SEC31L1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The W985A described in the publication is actually a W995A mutation, it was mis-named.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-Sec31a W985A #790 was a gift from Ayano Satoh (Addgene plasmid # 42122 ; http://n2t.net/addgene:42122 ; RRID:Addgene_42122)

  • For your References section:

    CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking. Koreishi M, Yu S, Oda M, Honjo Y, Satoh A. PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 2013 Jan 18. 10.1371/journal.pone.0054382 PubMed 23349870
Related items: