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Addgene

FLAG-Sec31a S527/799/1165A #31#907
(Plasmid #42121)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42121 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFLAG-CMV
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sec31a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3700
  • Mutation
    S527A S799A S1165A
  • Entrez Gene
    SEC31A (a.k.a. ABP125, ABP130, HPBKS, HSPC275, HSPC334, NEDSOSB, SEC31L1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-Sec31a S527/799/1165A #31#907 was a gift from Ayano Satoh (Addgene plasmid # 42121 ; http://n2t.net/addgene:42121 ; RRID:Addgene_42121)

  • For your References section:

    CK2 phosphorylates Sec31 and regulates ER-To-Golgi trafficking. Koreishi M, Yu S, Oda M, Honjo Y, Satoh A. PLoS One. 2013;8(1):e54382. doi: 10.1371/journal.pone.0054382. Epub 2013 Jan 18. 10.1371/journal.pone.0054382 PubMed 23349870
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