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pLuc-CDS-m215-1+2
(Plasmid #42103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42103 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK-1
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3565
  • Total vector size (bp) 6940
  • Modifications to backbone
    The stop codon of Renilla luciferase was removed, and a SalI restriction site was added by PCR.
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZEB1
  • Species
    H. sapiens (human)
  • Mutation
    Silent mutations were introduced in two seed-complementary sites of dsEcad215 antisense strand (215a-1 and 215a-2).
  • GenBank ID
    NM_030751.4
  • Entrez Gene
    ZEB1 (a.k.a. AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A)
  • Promoter SV40
  • Tag / Fusion Protein
    • Renilla luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CGCAACTACAACGCCTACCT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLuc-CDS-m215-1+2 was a gift from Kumiko Ui-Tei (Addgene plasmid # 42103 ; http://n2t.net/addgene:42103 ; RRID:Addgene_42103)

  • For your References section:

    E-cadherin is transcriptionally activated via suppression of ZEB1 transcriptional repressor by small RNA-mediated gene silencing. Mazda M, Nishi K, Naito Y, Ui-Tei K. PLoS One. 2011;6(12):e28688. doi: 10.1371/journal.pone.0028688. Epub 2011 Dec 21. 10.1371/journal.pone.0028688 PubMed 22205962
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