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pLenti-SPA2G231V
(Plasmid #42079)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42079 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6/V5/GW/lacZ
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 10127
  • Total vector size (bp) 10877
  • Vector type
    Mammalian Expression, Bacterial Expression, Lentiviral ; pLenti
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Surfactant Protein -A2
  • Alt name
    SPA2,SFTPA2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    750
  • Mutation
    Changed Glycine 231 to Valine
  • GenBank ID
    NM_001098668.2
  • Entrez Gene
    SFTPA2 (a.k.a. COLEC5, ILD2, PSAP, PSP-A, PSPA, SFTP1, SFTPA2B, SP-2A, SP-A, SPA2, SPAII)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The V5 tag has been inserted internally to the insert after ~21st amino acid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-SPA2G231V was a gift from Christine Garcia (Addgene plasmid # 42079 ; http://n2t.net/addgene:42079 ; RRID:Addgene_42079)

  • For your References section:

    Mutant surfactant A2 proteins associated with familial pulmonary fibrosis and lung cancer induce TGF-beta1 secretion. Maitra M, Cano CA, Garcia CK. Proc Natl Acad Sci U S A. 2012 Dec 18;109(51):21064-9. doi: 10.1073/pnas.1217069110. Epub 2012 Dec 5. 10.1073/pnas.1217069110 PubMed 23223528
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