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Addgene

pUAS-CFP-MEK
(Plasmid #42077)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42077 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pUAST
  • Backbone size w/o insert (bp) 8904
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Drosophila MEK ortholog
  • Alt name
    Dsor1
  • Alt name
    dmek
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    Dsor1 (a.k.a. Dmel_CG15793, CG15793, D-MEK, D-MEK/Dsor, D-Mek, D-SOR, D-Sor, D-mek, D-sor, D-sor-1, DMEK-1, DRODSOR1, DSOR, DSOR1, DSORT, DSor, DSor1, Dmek, Dmel\CG15793, Dsor, EK1-1, MAPKK, MEK, MEK/Dsor1, MEK1/2, Mek, Mek1/2, SOR, Sor, Su(Raf)34B, dMEK, dMek, dSor, dSor1, dsor1, mek, sor, sor/MEK1)
  • Promoter Hsp70
  • Tag / Fusion Protein
    • CFP (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full–length cDNA clone for MEK (Dsor1) was obtained from the Berkeley Drosophila Genome Project (BDGP) clone collection LD41207

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUAS-CFP-MEK was a gift from Norbert Perrimon (Addgene plasmid # 42077 ; http://n2t.net/addgene:42077 ; RRID:Addgene_42077)
  • For your References section:

    A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman A, Perrimon N. Nature. 2006 Nov 9;444(7116):230-4. Epub 2006 Nov 1. 10.1038/nature05280 PubMed 17086199