pUAS-CFP-MEK
(Plasmid
#42077)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 42077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUAST
- Backbone size w/o insert (bp) 8904
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDrosophila MEK ortholog
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Alt nameDsor1
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Alt namedmek
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SpeciesD. melanogaster (fly)
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Entrez GeneDsor1 (a.k.a. Dmel_CG15793, CG15793, D-MEK, D-MEK/Dsor, D-Mek, D-SOR, D-Sor, D-mek, D-sor, D-sor-1, DMEK-1, DRODSOR1, DSOR, DSOR1, DSORT, DSor, DSor1, Dmek, Dmel\CG15793, Dsor, EK1-1, MAPKK, MEK, MEK/Dsor1, MEK1/2, Mek, Mek1/2, SOR, Sor, Su(Raf)34B, dMEK, dMek, dSor, dSor1, dsor1, mek, sor, sor/MEK1)
- Promoter Hsp70
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Tag
/ Fusion Protein
- CFP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pCasper-hs
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Full–length cDNA clone for MEK (Dsor1) was obtained from the Berkeley Drosophila Genome Project (BDGP) clone collection LD41207
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAS-CFP-MEK was a gift from Norbert Perrimon (Addgene plasmid # 42077 ; http://n2t.net/addgene:42077 ; RRID:Addgene_42077) -
For your References section:
A functional RNAi screen for regulators of receptor tyrosine kinase and ERK signalling. Friedman A, Perrimon N. Nature. 2006 Nov 9;444(7116):230-4. Epub 2006 Nov 1. 10.1038/nature05280 PubMed 17086199