pUAS-ERK-YFP
(Plasmid #42076)

• Depositing Lab: Norbert Perrimon
• Publication: Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub 2006 Nov 1.

Backbone

• Vector backbone: pUAST
• Backbone size w/o insert (bp): 8904
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Drosophila ERK ortholog
• Alt name: rolled
• Alt name: rl
• Species: D. melanogaster (fly)
• Entrez Gene: rl (a.k.a. Dmel_CG12559, 12559, BcDNA:RE08694, CG12559, CG18732, CT34260, CT39192, D-ERK, DERK, DERK-A, DmERK, DmERK-A, DmERKA, DmErk, DmMAPK, Dmel\CG12559, DpERK, DpErk, Dsor2, E(sina)7, EC2-1, EK2-1, ERK, ERK-A, ERK1, ERK1/2, ERKA, ERKa, EY2-2, Erk, Erk/Map kinase, Erk1, Erk1/2, ErkA, GroupII, MAP-k, MAPK, MapK, Mapk, RL, Rl, Rolled, SEM, SR2-1, Sem, Su(Raf)2B, dERK, dErk, dm-dpERK, dp-ERK, dpERK, dpERK1, dpERk, dpErk, dpMAPK, erk, l(2)41Ac, l(2R)EMS45-39, mapk, p-ERK, p-Erk, pERK, pMAPK, pMapK, rl/ERK, rl/MAPK, rll, sem)
• Promoter: Hsp70
• Tag / Fusion Protein:
  • YFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCasper-hs
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full–length cDNA clone for ERK (rl) was obtained from the Berkeley Drosophila Genome Project (BDGP) clone collection RE08694