slpr genomic fragment
(Plasmid #42074)

• Depositing Lab: Norbert Perrimon
• Publication: Stronach et al Genes Dev. 2002 Feb 1;16(3):377-87.

Backbone

• Vector backbone: pCaSpeR4
• Backbone size w/o insert (bp): 7862
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: slpr genomic fragment
• Alt name: slipper
• Species: D. melanogaster (fly)
• Entrez Gene: slpr (a.k.a. Dmel_CG2272, CG2272, D-MLK, Dmel\CG2272, JNKKK, MLK, MLK2, Mlk2, Slpr, dMLK, dMLK2)
• Promoter: Hsp70

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pCasper-F
• 3′ sequencing primer: pCasper-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The slpr genomic rescue construct was generated by isolating a HindIII fragment of ∼14 kb from the P1 clone DS08402, including the MLK gene, and a neighboring annotated geneCG15339. This fragment was cloned transiently into pBluescript (Stratagene) and then moved into pCaSpeR4 vector for subsequent purification and germ-line transformation.