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Addgene

hs-leonardo
(Plasmid #42065)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42065 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCaSpeR-hs
  • Backbone size w/o insert (bp) 8780
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    leonardo
  • Alt name
    leo
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    780
  • Entrez Gene
    14-3-3zeta (a.k.a. Dmel_CG17870, 14-3-3, 14-3-3 zeta, 14-3-3-Leo, 14-3-3-zeta, 14-3-3ZETA, 14-3-3leo, 14-3-3z, 14.3.3, 1433Z, 2G1, 4-3-3 zeta, 5.11, 549, BEST:GH05075, CG17870, D14-3-3, D14-3-3zeta, Dmel\CG17870, K, LEO, Leo, PAR-5, PAR5, Par-5, d14-3-3zeta, l(2)07103, l(2)46CFe, l(2)46Ee, leo, par-5)
  • Promoter Hsp70

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pCasper-hs
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hs-leonardo was a gift from Norbert Perrimon (Addgene plasmid # 42065 ; http://n2t.net/addgene:42065 ; RRID:Addgene_42065)

  • For your References section:

    The Drosophila 14-3-3 protein Leonardo enhances Torso signaling through D-Raf in a Ras 1-dependent manner. Li W, Skoulakis EM, Davis RL, Perrimon N. Development. 1997 Oct;124(20):4163-71. PubMed 9374412
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