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Addgene

sugarless cDNA
(Plasmid #42063)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42063 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNB40
  • Backbone size w/o insert (bp) 7200

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sugarless
  • Alt name
    sgl
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    sgl (a.k.a. Dmel_CG10072, 8310, BG01214, CG10072, Dmel\CG10072, UDP-GlcDH, UDPGDH, XIII, kiwi, l(3)05007, l(3)08310, l(3)10503, l(3)65CEd, l(3)SG9, ska, sl)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sugarless cDNA was a gift from Norbert Perrimon (Addgene plasmid # 42063 ; http://n2t.net/addgene:42063 ; RRID:Addgene_42063)

  • For your References section:

    The Drosophila sugarless gene modulates Wingless signaling and encodes an enzyme involved in polysaccharide biosynthesis. Hacker U, Lin X, Perrimon N. Development. 1997 Sep;124(18):3565-73. PubMed 9342049
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