UAS-akkordion dNhe
(Plasmid
#41976)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41976 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUAST
- Backbone size w/o insert (bp) 8904
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDRhoGEF2
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Alt namedakkordeon (akk)
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SpeciesD. melanogaster (fly)
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GenBank IDAF031930
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Entrez GeneRhoGEF2 (a.k.a. Dmel_CG9635, CG9635, DRhoGEF, DRhoGEF2, Dmel\CG9635, DrhoGEF2, Gef2, RG2, Rho-GEF2, RhoGEF, RhoGF2, RhoGef2, Su(Rho1)2B, T2, dRhoGEF, dRhoGEF2, l(2)04291, rhoGEF2, rhogef2, shar)
- Promoter Hsp70
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer pCasper-hs
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UAS-akkordion dNhe was a gift from Norbert Perrimon (Addgene plasmid # 41976 ; http://n2t.net/addgene:41976 ; RRID:Addgene_41976) -
For your References section:
DRhoGEF2 encodes a member of the Dbl family of oncogenes and controls cell shape changes during gastrulation in Drosophila. Hacker U, Perrimon N. Genes Dev. 1998 Jan 15;12(2):274-84. 10.1101/gad.12.2.274 PubMed 9436986