pAO139 (Apr LEU2 mrcl-AQ)
(Plasmid
#41924)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS415
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 6021
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMediator of the Replication Checkpoint
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Alt nameMRC1
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SpeciesS. cerevisiae (budding yeast)
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MutationMRC1AQ
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Sal1 (unknown if destroyed)
- 3′ cloning site Pac1 (unknown if destroyed)
- 5′ sequencing primer M13_forward20_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is a D304N mutation in LEU, relative to aa1 in LEU, it is not thought to affect function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAO139 (Apr LEU2 mrcl-AQ) was a gift from Stephen Elledge (Addgene plasmid # 41924 ; http://n2t.net/addgene:41924 ; RRID:Addgene_41924) -
For your References section:
Mrc1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad53. Osborn AJ, Elledge SJ. Genes Dev. 2003 Jul 15;17(14):1755-67. 10.1101/gad.1098303 PubMed 12865299