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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41915 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE
- Backbone size w/o insert (bp) 8000
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCUL4B
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Alt nameENSG00000158290
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SpeciesH. sapiens (human)
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GenBank IDNM_003588
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Entrez GeneCUL4B (a.k.a. CUL-4B, MRXHF2, MRXS15, MRXSC, SFM2)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE DN Cullin4B was a gift from Stephen Elledge (Addgene plasmid # 41915 ; http://n2t.net/addgene:41915 ; RRID:Addgene_41915) -
For your References section:
Global identification of modular cullin-RING ligase substrates. Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ. Cell. 2011 Oct 14;147(2):459-74. doi: 10.1016/j.cell.2011.09.019. Epub 2011 Sep 29. 10.1016/j.cell.2011.09.019 PubMed 21963094