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Addgene

LPC-flag-REST-WT
(Plasmid #41903)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41903 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPC
  • Backbone size w/o insert (bp) 5763
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RE1-silencing transcription factor
  • Alt name
    NRSF
  • Species
    H. sapiens (human)
  • Entrez Gene
    REST (a.k.a. DFNA27, GINGF5, HGF5, NRSF, WT6, XBR)
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LPC-flag-REST-WT was a gift from Stephen Elledge (Addgene plasmid # 41903 ; http://n2t.net/addgene:41903 ; RRID:Addgene_41903)

  • For your References section:

    SCFbeta-TRCP controls oncogenic transformation and neural differentiation through REST degradation. Westbrook TF, Hu G, Ang XL, Mulligan P, Pavlova NN, Liang A, Leng Y, Maehr R, Shi Y, Harper JW, Elledge SJ. Nature. 2008 Mar 20;452(7185):370-4. doi: 10.1038/nature06780. 10.1038/nature06780 PubMed 18354483
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