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Addgene

pBABE HA Chk2 KD, dominant negative chk2
(Plasmid #41902)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41902 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Chk2
  • Species
    H. sapiens (human)
  • Mutation
    D347A
  • Entrez Gene
    CHEK2 (a.k.a. CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, TPDS4, hCds1)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE HA Chk2 KD, dominant negative chk2 was a gift from Stephen Elledge (Addgene plasmid # 41902 ; http://n2t.net/addgene:41902 ; RRID:Addgene_41902)

  • For your References section:

    ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER 3rd, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ. Science. 2007 May 25;316(5828):1160-6. 10.1126/science.1140321 PubMed 17525332
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