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pKT127-SEP
(Plasmid #41899)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41899 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKT127
  • Backbone manufacturer
    Kurt S. Thorn
  • Backbone size (bp) 4894
  • Modifications to backbone
    Replaced yeGFP with superecliptic GFP (SEP)
  • Vector type
    Yeast Expression
  • Selectable markers
    G418
  • Tag / Fusion Protein
    • superecliptic GFP (SEP) (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pKT127-SEP, used for creating in-frame C-terminal chromosomal fusions of a gene of interest with SEP, was created by removing yeGFP from pKT127 (http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/pKT127.html) by restriction digestion with PacI/AscI and replacing it with PacI/AscI-digested SEP generated by PCR from template plasmid pGM87

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKT127-SEP was a gift from Dan Gottschling (Addgene plasmid # 41899 ; http://n2t.net/addgene:41899 ; RRID:Addgene_41899)

  • For your References section:

    An early age increase in vacuolar pH limits mitochondrial function and lifespan in yeast. Hughes AL, Gottschling DE. Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 2012 Nov 21. 10.1038/nature11654 PubMed 23172144
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