pAG306-GPD-VPH2 chr I
(Plasmid
#41897)
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PurposeYeast expression of VPH2.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41897 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAG306-GPD-ccdB chr I
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Backbone manufacturerDan Gottschling (Addgene plasmid# 41894)
- Backbone size w/o insert (bp) 7882
- Total vector size (bp) 6878
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Modifications to backboneInserted VPH2 using LR clonase
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVPH2
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Alt nameVph2p
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SpeciesS. cerevisiae (budding yeast)
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GenBank ID
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Entrez GeneVPH2 (a.k.a. YKL119C, CLS10, VMA12)
- Promoter GPD
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer pBluescriptSK_primer
- 3′ sequencing primer pBluescriptKS_primer (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byVPH2 from donor Gateway plasmid pDONR221-VPH2 Harvard Institute of Proteomics (HIP) accession ScCD00012560
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG306-GPD-VPH2 chr I was a gift from Dan Gottschling (Addgene plasmid # 41897 ; http://n2t.net/addgene:41897 ; RRID:Addgene_41897) -
For your References section:
An early age increase in vacuolar pH limits mitochondrial function and lifespan in yeast. Hughes AL, Gottschling DE. Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 2012 Nov 21. 10.1038/nature11654 PubMed 23172144