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pAG306-GPD-VMA1 chr I
(Plasmid #41896)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41896 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAG306-GPD-ccdB chr I
  • Backbone manufacturer
    Dan Gottschling (Addgene plasmid# 41894)
  • Backbone size w/o insert (bp) 7882
  • Total vector size (bp) 9446
  • Modifications to backbone
    Inserted VMA1 using LR clonase
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VMA1
  • Alt name
    TFP1
  • Alt name
    Vma1p
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    Contains silent mutation at 342A-G and L87S mutation in VMA1; 2144A-G mutation in SceI intein
  • GenBank ID
    NM_001180245
  • Entrez Gene
    VMA1 (a.k.a. YDL185W, CLS8, TFP1)
  • Promoter GPD

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer pBluescriptSK_primer
  • 3′ sequencing primer pBluescriptKS_primer
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    VMA1 from donor Gateway plasmid pDONR221-VMA1, Harvard Institute of Proteomics (HIP) accession ScCD00023712

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAG306-GPD-VMA1 chr I was a gift from Dan Gottschling (Addgene plasmid # 41896 ; http://n2t.net/addgene:41896 ; RRID:Addgene_41896)

  • For your References section:

    An early age increase in vacuolar pH limits mitochondrial function and lifespan in yeast. Hughes AL, Gottschling DE. Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 2012 Nov 21. 10.1038/nature11654 PubMed 23172144
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