pAG306-GPD-empty chr I
(Plasmid
#41895)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41895 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepAG306-GPD-ccdB chr I
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Backbone manufacturerDan Gottschling (Addgene plasmid# 41894)
- Backbone size (bp) 7882
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Modifications to backboneRemoved ccdB by digestion with HindIII and SpeI followed by filling in with T4 DNA polymerase and blunt end ligation
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Vector typeYeast Expression
- Promoter GPD
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG306-GPD-empty chr I was a gift from Dan Gottschling (Addgene plasmid # 41895 ; http://n2t.net/addgene:41895 ; RRID:Addgene_41895) -
For your References section:
An early age increase in vacuolar pH limits mitochondrial function and lifespan in yeast. Hughes AL, Gottschling DE. Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 2012 Nov 21. 10.1038/nature11654 PubMed 23172144
Map uploaded by the depositor.
